Application and Prospect of Nanopore Sequencing Technology in Etiological Diagnosis of Blood Stream Infection.
10.3881/j.issn.1000-503X.14995
- Author:
Wei GUO
1
;
Shuai-Hua FAN
1
;
Peng-Cheng DU
2
;
Jun GUO
1
Author Information
1. Department of Respiratory and Critical Care Medicine,Beijing Tsinghua Changgung Hospital Affiliated to Tsinghua University,School of Clinical Medicine,Tsinghua University,Beijing 102218,China.
2. Beijing Key Laboratory of Emerging Infectious Diseases,Institute of Infectious Diseases,Beijing Ditan Hospital Affiliated to Capital Medical University,Beijing 100015,China.
- Publication Type:Journal Article
- Keywords:
blood stream infection;
high-throughput nucleotide sequencing;
nanopore sequencing
- MeSH:
Humans;
Nanopore Sequencing;
Sepsis/diagnosis*;
Bacteremia/microbiology*;
Bacteria;
Blood Culture/methods*
- From:
Acta Academiae Medicinae Sinicae
2023;45(2):317-321
- CountryChina
- Language:Chinese
-
Abstract:
Blood stream infection (BSI),a blood-borne disease caused by microorganisms such as bacteria,fungi,and viruses,can lead to bacteremia,sepsis,and infectious shock,posing a serious threat to human life and health.Identifying the pathogen is central to the precise treatment of BSI.Traditional blood culture is the gold standard for pathogen identification,while it has limitations in clinical practice due to the long time consumption,production of false negative results,etc.Nanopore sequencing,as a new generation of sequencing technology,can rapidly detect pathogens,drug resistance genes,and virulence genes for the optimization of clinical treatment.This paper reviews the current status of nanopore sequencing technology in the diagnosis of BSI.
