Mitochondrial genome sequence characteristics and phylogenetic analysis of <i>Schizothorax argentatus</i>.

Yuping Liu; Jianyong HU; Zijun Ning; Peiyi Xiao; Tianyan Yang

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Mitochondrial genome sequence characteristics and phylogenetic analysis of Schizothorax argentatus.

Author: Yuping LIU ¹ ; Jianyong HU ² ; Zijun NING ¹ ; Peiyi XIAO ¹ ; Tianyan YANG ¹
Author Information

1. Fisheries College, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China.
2. Xinjiang Fishery Research Institute, Urumqi 830000, Xinjiang, China.
Publication Type:Journal Article
Keywords: Schizothorax argenteus; mitochondrial genome; phylogenetics; sequence analysis
MeSH: Animals; Genome, Mitochondrial/genetics*; Phylogeny; Sequence Analysis, DNA; Cyprinidae/genetics*; RNA, Transfer/genetics*; DNA, Mitochondrial/genetics*; Genes, Mitochondrial
From: Chinese Journal of Biotechnology 2023;39(7):2965-2985
CountryChina
Language:Chinese
Abstract: Schizothorax argentatus that only distributes in the Ili River basin in Xinjiang is one of the rare and endangered species of schizothorax in China, thus has high scientific and economic values. In this study, the complete mitochondrial genome sequence of S. argenteus with a length of 16 580 bp was obtained by high-throughput sequencing. The gene compositions and arrangement were similar to those of typical vertebrates. It contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a non-coding region (D-loop). The nucleotide compositions were A (30.25%), G (17.28%), C (27.20%), and T (25.27%), respectively, showing obvious AT bias and anti-G bias. Among the tRNA genes, only tRNA-Ser^(GCU) could not form a typical cloverleaf structure due to the lack of dihydrouracil arm. The AT-skew and GC-skew values of the ND6 gene were fluctuating the most, suggesting that the gene may experience different selection and mutation pressures from other genes. The mitochondrial control region of S. argenteus contained three different domains, i.e., termination sequence region (ETAS), central conserved region (CSB-F, CSB-E, CSB-D, and CSB-B), and conserved sequence region (CSB1, CSB2, and CSB3). The conserved sequence fragment TT (AT) nGTG, which was ubiquitous in Cypriniformes, was identified at about 50 bp downstream CSB3. Phylogenetic relationships based on the complete mitochondrial genome sequence of 28 Schizothorax species showed that S. argenteus had differentiated earlier and had a distant relationship with other species, which may be closely related to the geographical location and the hydrological environment where it lives.