Analysis of the chloroplast genome of <i>Incarvillea younghusbandii</i> Sprague.

Yaying Zhang; Wanyao Jiao; Wenrui Jiao; Tianle Qiao; Zhiyang SU; Shuo Feng

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Analysis of the chloroplast genome of Incarvillea younghusbandii Sprague.

Author: Yaying ZHANG ¹ ; Wanyao JIAO ¹ ; Wenrui JIAO ¹ ; Tianle QIAO ¹ ; Zhiyang SU ¹ ; Shuo FENG ¹
Author Information

1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, Qinghai, China.
Collective Name:Yixizhuoma
Publication Type:Journal Article
Keywords: Incarvillea younghusbandii; codon usage bias; complete chloroplast genome; estimated divergence time; phylogenetic; simple sequence repeats (SSR)
MeSH: Phylogeny; Molecular Sequence Annotation; Genome, Chloroplast; Sequence Analysis, DNA; Whole Genome Sequencing
From: Chinese Journal of Biotechnology 2023;39(7):2954-2964
CountryChina
Language:Chinese
Abstract: Incarvillea younghusbandii Sprague is a traditional tonic herb. The roots are used as herbal medicine for nourishing and strengthening, as well as treating postpartum milk deficiency and weakness. In this study, the chloroplast genome of I. younghusbandii was sequenced and assembled by the high-throughput sequencing technology. The sequence characteristics, sequence repeats, codon usage bias, phylogenetic relationships and estimated divergence time of I. younghusbandii were analyzed. The 159 323 bp sequence contained a large single copy (80 197 bp), a small single copy (9 030 bp) and two inverted repeat sequences (35 048 bp). It contained 120 genes, including 77 protein coding genes, 8 ribosomal RNA genes and 35 transfer RNA genes. AAA was the most frequent codon in the chloroplast coding sequence of I. younghusbandii. A total of 42 simple sequence repeats were identified in the chloroplast genome. Phylogenetic analysis revealed I. younghusbandii was mostly like its taxonomically close relative Incarvillea compacta. The divergence between I. younghusbandii and I. compacta was dated to 4.66 million years ago. This study was significant for the scientific conservation and development of resources related to I. compacta. It also provides a basic genetic resource for the subsequent species identification of the genus Incarvillea, and the population genetic diversity study of Bignoniaceae.