Soybean GmGolS2-2 improves drought resistance of transgenic tobacco.
- Author:
Haiwei YU
1
;
Shuang QIU
1
;
Jun ZHANG
2
;
Shanshan LI
1
;
Tianguo SUN
1
;
Tianyi MA
1
;
Yan ZHAO
1
;
Xu ZHAO
3
;
Ying ZHAI
1
Author Information
- Publication Type:Journal Article
- Keywords: drought resistant gene; galactinol synthase; genetic transformation; soybean; tobacco
- MeSH: Drought Resistance; Tobacco/genetics*; Soybeans/genetics*; Plant Proteins/metabolism*; Plants, Genetically Modified/genetics*; Stress, Physiological/genetics*; Droughts; Gene Expression Regulation, Plant
- From: Chinese Journal of Biotechnology 2023;39(7):2762-2771
- CountryChina
- Language:Chinese
- Abstract: Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean GmGolS2-2 gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A GmGolS2-2 gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). GmGolS2-2 was linked to the plant expression vector pRI101 by restriction enzyme sites Nde Ⅰ and EcoR Ⅰ, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three GmGolS2-2 transgenic tobacco plants were obtained. The growth status of GmGolS2-2 transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of GmGolS2-2 increased the expression of stress-related genes NtERD10C and NtAQP1 in transgenic tobacco. The above results indicated that GmGolS2-2 improved drought resistance of transgenic tobacco.
