Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea.

Limin Yang; Junhong Wang; Mingguo XU; Hu Wang; Xiaojuan Zhang; Wenjun Liu; Chuangfu Chen

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Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea.

Author: Limin YANG ¹ ; Junhong WANG ² ; Mingguo XU ¹ ; Hu WANG ³ ; Xiaojuan ZHANG ⁴ ; Wenjun LIU ⁵ ; Chuangfu CHEN ¹
Author Information

1. Key Laboratory of Zoonoses, College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China.
2. Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
3. Liaoning Agricultural Development Service Center, Shenyang 110003, Liaoning, China.
4. Panjin Modern Agriculture Development Center, Panjin 124000, Liaoning, China.
5. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Publication Type:Journal Article
Keywords: cellular immune response; humoral immune response; mRNA vaccine; porcine epidemic diarrhea; receptor-binding region
MeSH: Pregnancy; Swine; Animals; Female; Mice; Antibodies, Viral; Swine Diseases/epidemiology*; Viral Vaccines/genetics*; Antibodies, Neutralizing; Vaccines, Attenuated; Diarrhea/veterinary*
From: Chinese Journal of Biotechnology 2023;39(7):2624-2633
CountryChina
Language:Chinese
Abstract: Porcine epidemic diarrhea (PED) is a highly contagious disease that causes high mortality in suckling piglets. Although several licensed inactivated and live attenuated vaccines were widely used, the infection rate remains high due to unsatisfactory protective efficacy. In this study, mRNA vaccine candidates against PED were prepared, and their immunogenicity was evaluated in mice and pregnant sows. The mRNA PED vaccine based on heterodimer of viral receptor binding region (RBD) showed good immunogenicity. It elicited robust humoral and cellular immune responses in mice, and the neutralizing antibody titer reached 1:300 after a single vaccination. Furthermore, it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows. This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.