Precision gene editing technologies based on CRISPR/Cas9: a review.

Author: Shan XUE ¹ ; Shuya WANG ¹ ; Li LIU ¹ ; Qiaofang ZHONG ² ; Zaiquan CHENG ¹ ; Suqin XIAO ²
Author Information

1. School of Agriculture, Yunnan University, Kunming 650504, Yunnan, China.
2. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China.
Publication Type:Journal Article
Keywords: CRISPR/Cas9; double base editor; prime editor; single base editor; transposon-associated editor
MeSH: Gene Editing; CRISPR-Cas Systems/genetics*; Mutation; Genome
From: Chinese Journal of Biotechnology 2023;39(7):2566-2578
CountryChina
Language:Chinese
Abstract: Gene editing technology is a genetic operation technology that can modify the DNA sequence at the genomic level. The precision gene editing technology based on CRISPR/Cas9 system is a gene editing technology that is easy to operate and widely used. Unlike the traditional CRISPR/Cas9 system, the precision gene editing technology can perform site-directed mutation of genes without DNA template. This review summarizes the recent development of precision gene editing technology based on CRISPR/Cas9, and prospects the challenges and opportunities of this technology.