Metabolic engineering of <i>Escherichia coli</i> for adipic acid production.

Jie Liu; Cong Gao; Xiulai Chen; Liang Guo; Wei Song; Jing WU; Wanqing Wei; Jia Liu; Liming Liu

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Metabolic engineering of Escherichia coli for adipic acid production.

Author: Jie LIU ¹ ; Cong GAO ¹ ; Xiulai CHEN ¹ ; Liang GUO ¹ ; Wei SONG ² ; Jing WU ² ; Wanqing WEI ¹ ; Jia LIU ¹ ; Liming LIU ¹
Author Information

1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
2. School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: Escherichia coli; adipic acid; fermentation optimization; metabolic engineering
MeSH: Escherichia coli/metabolism*; Metabolic Engineering; Bioreactors; Fermentation; Adipates/metabolism*
From: Chinese Journal of Biotechnology 2023;39(6):2375-2389
CountryChina
Language:Chinese
Abstract: Adipic acid is a high-value-added dicarboxylic acid which is primarily used in the production of nylon-66 for manufacturing polyurethane foam and polyester resins. At present, the biosynthesis of adipic acid is hampered by its low production efficiency. By introducing the key enzymes of adipic acid reverse degradation pathway into a succinic acid overproducing strain Escherichia coli FMME N-2, an engineered E. coli JL00 capable of producing 0.34 g/L adipic acid was constructed. Subsequently, the expression level of the rate-limiting enzyme was optimized and the adipic acid titer in shake-flask fermentation increased to 0.87 g/L. Moreover, the supply of precursors was balanced by a combinatorial strategy consisting of deletion of sucD, over-expression of acs, and mutation of lpd, and the adipic acid titer of the resulting E. coli JL12 increased to 1.51 g/L. Finally, the fermentation process was optimized in a 5 L fermenter. After 72 h fed-batch fermentation, adipic acid titer reached 22.3 g/L with a yield of 0.25 g/g and a productivity of 0.31 g/(L·h). This work may serve as a technical reference for the biosynthesis of various dicarboxylic acids.