Construction of multi-enzyme cascade reactions and its application in the synthesis of bifunctional chemicals.

Author: Jumou LI ¹ ; Kun SHI ¹ ; Zhijun ZHANG ¹ ; Jianhe XU ¹ ; Huilei YU ¹
Author Information

1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Publication Type:Journal Article
Keywords: bifunctional chemicals; biocatalysis; coenzyme regeneration; construction strategies; multi-enzyme cascades
MeSH: Amino Acids; Biocatalysis; Amino Alcohols; Coenzymes/metabolism*; Diamines
From: Chinese Journal of Biotechnology 2023;39(6):2158-2189
CountryChina
Language:Chinese
Abstract: The synthesis of fine chemicals using multi-enzyme cascade reactions is a recent hot research topic in the field of biocatalysis. The traditional chemical synthesis methods were replaced by constructing in vitro multi-enzyme cascades, then the green synthesis of a variety of bifunctional chemicals can be achieved. This article summarizes the construction strategies of different types of multi-enzyme cascade reactions and their characteristics. In addition, the general methods for recruiting enzymes used in cascade reactions, as well as the regeneration of coenzyme such as NAD(P)H or ATP and their application in multi-enzyme cascade reactions are summarized. Finally, we illustrate the application of multi-enzyme cascades in the synthesis of six bifunctional chemicals, including ω-amino fatty acids, alkyl lactams, α, ω-dicarboxylic acids, α, ω-diamines, α, ω-diols, and ω-amino alcohols.