A new biosynthesis route for production of 5-aminovalanoic acid, a biobased plastic monomer.
- Author:
Yaqi KANG
1
;
Ruoshi LUO
1
;
Fanzhen LIN
1
;
Jie CHENG
1
;
Zhen ZHOU
2
;
Dan WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: 5-aminovaleric acid; L-lysine; metabolic engineering; synthetic route
- MeSH: Nylons; Lysine/metabolism*; Hydrogen Peroxide/metabolism*; Metabolic Engineering; Plastics/metabolism*; Fermentation; Escherichia coli/metabolism*; Aminocaproates/metabolism*
- From: Chinese Journal of Biotechnology 2023;39(5):2070-2080
- CountryChina
- Language:Chinese
- Abstract: 5-aminovalanoic acid (5AVA) can be used as the precursor of new plastics nylon 5 and nylon 56, and is a promising platform compound for the synthesis of polyimides. At present, the biosynthesis of 5-aminovalanoic acid generally is of low yield, complex synthesis process and high cost, which hampers large-scale industrial production. In order to achieve efficient biosynthesis of 5AVA, we developed a new pathway mediated by 2-keto-6-aminohexanoate. By combinatory expression of L-lysine α-oxidase from Scomber japonicus, α-ketoacid decarcarboxylase from Lactococcus lactis and aldehyde dehydrogenase from Escherichia coli, the synthesis of 5AVA from L-lysine in Escherichia coli was achieved. Under the initial conditions of glucose concentration of 55 g/L and lysine hydrochloride of 40 g/L, the final consumption of 158 g/L glucose and 144 g/L lysine hydrochloride, feeding batch fermentation to produce 57.52 g/L of 5AVA, and the molar yield is 0.62 mol/mol. The new 5AVA biosynthetic pathway does not require ethanol and H2O2, and achieved a higher production efficiency as compared to the previously reported Bio-Chem hybrid pathway mediated by 2-keto-6-aminohexanoate.
