Isolation and identification of a polyester-polyurethane degrading bacterium Bacillus altitudinis YX8-1.
- Author:
Caiting ZENG
1
;
Junbin JI
1
;
Fanghui DING
1
;
Zhoukun LI
1
;
Hui CAO
1
;
Zhongli CUI
1
;
Xin YAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Bacillus altitudinis; biodegradation; plastic waste recycling; polyurethane plastics
- MeSH: Polyurethanes/chemistry*; Polyesters/chemistry*; Chromatography, Liquid; Phylogeny; Tandem Mass Spectrometry; Bacteria/metabolism*; Biodegradation, Environmental
- From: Chinese Journal of Biotechnology 2023;39(5):1976-1986
- CountryChina
- Language:Chinese
- Abstract: Although polyurethane (PUR) plastics play important roles in daily life, its wastes bring serious environmental pollutions. Biological (enzymatic) degradation is considered as an environmentally friendly and low-cost method for PUR waste recycling, in which the efficient PUR-degrading strains or enzymes are crucial. In this work, a polyester PUR-degrading strain YX8-1 was isolated from the surface of PUR waste collected from a landfill. Based on colony morphology and micromorphology observation, phylogenetic analysis of 16S rDNA and gyrA gene, as well as genome sequence comparison, strain YX8-1 was identified as Bacillus altitudinis. The results of high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed that strain YX8-1 was able to depolymerize self-synthesized polyester PUR oligomer (PBA-PU) to produce a monomeric compound 4, 4'-methylene diphenylamine. Furthermore, strain YX8-1 was able to degrade 32% of the commercialized polyester PUR sponges within 30 days. This study thus provides a strain capable of biodegradation of PUR waste, which may facilitate the mining of related degrading enzymes.
