Effect of naringenin on the anti-inflammatory, vascularization, and osteogenesis differentiation of human periodontal ligament stem cells via the stromal cell-derived factor 1/C-X-C motif chemokine receptor 4 signaling axis stimulated by lipopolysaccharide.
10.7518/hxkq.2023.2022293
- Author:
Shenghong LI
1
;
Shiyuan PENG
1
;
Xiaoling LUO
2
;
Yipei WANG
3
;
Xiaomei XU
1
Author Information
1. Dept. of Orthodontics, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China.
2. Dept. of Stomatology, The People's Hospital of Jianyang City, Jianyang 641400, China.
3. Dept. of Stomatology, Zigong First People's Hospital, Zigong 643000, China.
- Publication Type:Journal Article
- Keywords:
anti-inflammatory;
inflammatory human periodontal ligament stem cells;
naringenin;
osteogenesis;
vascularization
- MeSH:
Humans;
Anti-Inflammatory Agents/pharmacology*;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Chemokine CXCL12;
Lipopolysaccharides/pharmacology*;
Osteogenesis;
Periodontal Ligament/metabolism*;
Receptors, Chemokine/metabolism*;
Stem Cells;
Interleukin-8/metabolism*
- From:
West China Journal of Stomatology
2023;41(2):175-184
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:This study aimed to investigate how naringenin (Nar) affected the anti-inflammatory, vascula-rization, and osteogenesis differentiation of human periodontal ligament stem cells (hPDLSCs) stimulated by lipopolysaccharide (LPS) and to preliminarily explore the underlying mechanism.
METHODS:Cell-counting kit-8 (CCK8), cell scratch test, and Transwell assay were used to investigate the proliferation and migratory capabilities of hPDLSCs. Alkaline phosphatase (ALP) staining, alizarin red staining, lumen-formation assay, enzyme-linked immunosorbent assay, quantitative timed polymerase chain reaction, and Western blot were used to measure the expression of osteopontin (OPN), Runt-related transcription factor 2 (RUNX2), vascular endothlial growth factor (VEGF), basic fibroblast growth factor (bFGF), von Willebrand factor (vWF), tumor necrosis factor-α (TNF-α), and interleukin (IL)-6.
RESULTS:We observed that 10 μmol/L Nar could attenuate the inflammatory response of hPDLSCs stimulated by 10 μg/mL LPS and promoted their proliferation, migration, and vascularization differentiation. Furthermore, 0.1 μmol/L Nar could effectively restore the osteogenic differentiation of inflammatory hPDLSCs. The effects of Nar's anti-inflammatory and promotion of osteogenic differentiation significantly decreased and inflammatory vascularization differentiation increased after adding AMD3100 (a specific CXCR4 inhibitor).
CONCLUSIONS:Nar demonstrated the ability to promote the anti-inflammatory, vascularization, and osteogenic effects of hPDLSCs stimulated by LPS, and the ability was associated with the stromal cell-derived factor/C-X-C motif chemokine receptor 4 signaling axis.
