Effect of graphene-oxide-modified osteon-like concentric microgrooved surface on the osteoclastic differentiation of macrophages.
10.7518/hxkq.2023.2022354
- Author:
Hong WANG
1
;
Qinglin WU
1
;
Yingzhen LAI
1
;
Yihuang CAI
1
Author Information
1. Dept. of Stomatology, Xiamen Medical College & Engineering Research Center of Fujian University for Stomatological Biomaterials, Xiamen 361023, China.
- Publication Type:Journal Article
- Keywords:
concentric microgroove;
graphene oxide;
macrophages;
osteoclastic differentiation;
osteomimetic
- MeSH:
Graphite/pharmacology*;
Titanium/pharmacology*;
Haversian System;
Macrophages;
Cell Differentiation;
Oxides/pharmacology*;
Surface Properties
- From:
West China Journal of Stomatology
2023;41(2):165-174
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:This study aimed to investigate the effect of new biomimetic micro/nano surfaces on the osteoclastic differentiation of RAW264.7 macrophages by simulating natural osteons for the design of concentric circular structures and modifying graphene oxide (GO).
METHODS:The groups were divided into smooth titanium surface group (SS), concentric microgrooved titanium surface group (CMS), and microgroove modified with GO group (GO-CMS). The physicochemical properties of the material surfaces were studied using scanning electron microscopy (SEM), contact-angle measurement, atomic force microscopy, X-ray photoelectron spectroscopy analysis, and Raman spectroscopy. The effect of the modified material surface on the cell biological behavior of RAW264.7 was investigated by cell-activity assay, SEM, and laser confocal microscopy. The effect on the osteoclastic differentiation of macrophages was investiga-ted by tartrate-resistant acid phosphatase (TRAP) immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR) experiments.
RESULTS:Macrophages were arranged in concentric circles along the microgrooves, and after modification with GO, the oxygen-containing groups on the surface of the material increased and hydrophilicity increased. Osteoclasts in the GO-CMS group were small in size and number and had the lowest TRAP expression. Although it promoted the proliferation of macrophages in the GO-CMS group, the expression of osteoclastic differentiation-related genes was lower than that in the SS group, and the difference was statistically significant (P<0.05).
CONCLUSIONS:Concentric circular microgrooves restricted the fusion of osteoclasts and the formation of sealing zones. Osteomimetic concentric microgrooves modified with GO inhibited the osteoclastic differentiation of RAW 264.7 macrophages.
