Effect of recombinant human fibroblast growth factor 21 on the mineralization of cementoblasts and its related mechanism.
10.7518/hxkq.2023.2022375
- Author:
Hao WU
1
;
Ying LI
1
;
Yuzhuo WANG
1
;
Jize YU
2
;
Xingfu BAO
1
;
Min HU
1
Author Information
1. Dept. of Orthodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China.
2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China.
- Publication Type:Journal Article
- Keywords:
cementoblasts;
cementum regeneration;
fibroblast growth factor 21;
transforming growth factor β/ bone morphogenetic protein signaling pathway
- MeSH:
Humans;
Rats;
Animals;
Dental Cementum;
Core Binding Factor Alpha 1 Subunit/metabolism*;
Cell Differentiation;
Bone Morphogenetic Proteins/metabolism*;
Transforming Growth Factor beta/pharmacology*
- From:
West China Journal of Stomatology
2023;41(2):140-148
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the effect of recombinant human fibroblast growth factor 21 (rhFGF21) on the proliferation and mineralization of cementoblasts and its mechanism.
METHODS:Hematoxylin eosin, immunohistochemical staining, and immunofluorescence were used to detect the expression and distribution of fibroblast growth factor 21 (FGF21) in rat periodontal tissues and cementoblasts (OCCM-30), separately. Cell Counting Kit-8 was used to detect the proliferation of OCCM-30 under treatment with rhFGF21. Alkaline phosphatase staining and Alizarin Red staining were used to detect the mineralization state of OCCM-30 after 3 and 7 days of mineralization induction. The transcription and protein expression of the osteogenic-related genes Runx2 and Osterix were detected by real-time quantitative polymerase chain reaction (PCR) and Western blot analysis. The expression levels of genes of transforming growth factor β (TGFβ)/bone morphogenetic protein (BMP) signaling pathway in OCCM-30 were detected through PCR array analysis.
RESULTS:FGF21 was expressed in rat periodontal tissues and OCCM-30. Although rhFGF21 had no significant effect on the proliferation of OCCM-30, treatment with 50 ng/mL rhFGF21 could promote the mineralization of OCCM-30 cells after 7 days of mineralization induction. The transcriptional levels of Runx2 and Osterix increased significantly at 3 days of mineralization induction and decreased at 5 days of mineralization induction. Western blot analysis showed that the protein expression levels of Runx2 and Osterix increased during mineralization induction. rhFGF21 up-regulated Bmpr1b protein expression in cells.
CONCLUSIONS:rhFGF21 can promote the mineralization ability of OCCM-30. This effect is related to the activation of the TGFβ/BMP signaling pathway.
