Genotyping Characteristics of Human Fecal <i>Escherichia coli</i> and Their Association with Multidrug Resistance in Miyun District, Beijing.

Wei Wei Zhang; Xiao Lin Zhu; Le Le Deng; Ya Jun Han; Zhuo Wei LI; Jin Long Wang; Yong Liang Chen; Ao Lin Wang; Er Li Tian; Bin Cheng; Lin Hua XU; Yi Cong Chen; Li Li Tian; Guang Xue HE

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Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing.

Author: Wei Wei ZHANG ^{1
,

2} ; Xiao Lin ZHU ³ ; Le Le DENG ⁴ ; Ya Jun HAN ⁴ ; Zhuo Wei LI ⁴ ; Jin Long WANG ⁵ ; Yong Liang CHEN ² ; Ao Lin WANG ² ; Er Li TIAN ² ; Bin CHENG ⁶ ; Lin Hua XU ⁷ ; Yi Cong CHEN ⁷ ; Li Li TIAN ² ; Guang Xue HE ⁴
Author Information

1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. Miyun District Center for Disease Control and Prevention, Beijing 101520, China.
3. Berger Bioengineering Co., Ltd, Shanghai 201401, China.
4. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
5. Xicheng District Commission of Health of Beijing Municipality, Beijing 100044, China.
6. Miyun District Hospital, Beijing 101520, China.
7. Hebei North University, Zhangjiakou 075000, Heibei, China.
Publication Type:Journal Article
Keywords: Antibiotic resistance genes; E. coli; Multidrug resistance; Random forest; Whole-genome sequencing
MeSH: Humans; Escherichia coli/genetics*; Escherichia coli Infections/epidemiology*; Multilocus Sequence Typing; Genotype; Beijing; Drug Resistance, Multiple, Bacterial/genetics*; Anti-Bacterial Agents/pharmacology*; Diarrhea; Microbial Sensitivity Tests
From: Biomedical and Environmental Sciences 2023;36(5):406-417
CountryChina
Language:English
Abstract: OBJECTIVE:To explore the genotyping characteristics of human fecal Escherichia coli( E. coli) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of E. coli in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.
METHODS:Over a period of 3 years, 94 E. coli strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).
RESULTS:This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).
CONCLUSION:We developed a random forest (RF) prediction model of TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.