Effects and mechanism of pterostilbene on wound healing in diabetic skin ulcer model rats
- VernacularTitle:紫檀芪对糖尿病性皮肤溃疡模型大鼠创面愈合的影响及机制
- Author:
Yakun LIU
1
,
2
;
Gang LI
2
;
Juan YAN
3
;
Huizhi XU
4
;
Yanjiang WU
2
;
Shuai YOU
2
;
Jingya NIU
4
;
Hongjian LI
4
;
Binggui ZHANG
1
Author Information
1. Clinical College of Integrated Traditional Chinese and Western Medicine，Hebei University of Traditional Chinese Medicine，Shijiazhuang 050200，China
2. Dept. of Anorectal，Shijiazhuang Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
3. Dept. of Pharmacy，the First Affiliated Hospital of Hebei North University，Hebei Zhangjiakou 075000，China
4. Dept. of General Anorectal，the Eighth People’s Hospital of Hebei Province，Shijiazhuang 050024，China
- Publication Type:Journal Article
- Keywords:
pterostilbene;
diabetic skin ulcer;
wound healing;
SRC/mitogen-activated protein kinase kinase/extracellular signal-
- From:
China Pharmacy
2023;34(16):1967-1971
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effects of pterostilbene （PTE） on wound healing in diabetic skin ulcer model rats and its mechanism. METHODS Ten SD rats were grouped into control group； after diabetic skin ulcer model of other rats was induced by giving high-fat and high-sugar diet+intraperitoneal injection of streptozotocin+cutting off the skin and subcutaneous tissue in the marked area of the back， model rats were randomly divided into model group， PTE low-dose group （40 mg/kg）， PTE high-dose group （80 mg/kg）， PTE high-dose+PP2 group （80 mg/kg PTE+2 mg/kg SRC inhibitor PP2）， with 10 rats in each group. On the second day after modeling， the rats in each drug group were intraperitoneally injected with corresponding drug solutions， while the rats in control group and model group were intraperitoneally injected with normal saline， once a day， for 14 consecutive days. The wound healing rate of rats in each group was measured on the 7th and 14th day of administration； the contents of interleukin-1β （IL-1β）， IL-6， tumor necrosis factor-α （TNF-α） and vascular endothelial growth factor （VEGF） in the serum of rats were detected； the pathological changes of wound granulation tissue were observed， and the expressions of SRC/mitogen-activated protein kinase kinase （MEK）/extracellular signal-regulated kinase （ERK） signaling pathway-related proteins in wound granulation tissue were detected. RESULTS Compared with control group， the wound healing rate， serum content of VEGF， the phosphorylation levels of SRC， MEK1/2 and ERK1/2 were decreased significantly （P＜0.05）， while serum contents of IL- 1β， IL-6 and TNF-α were increased significantly （P＜0.05）； there was obvious infiltration of inflammatory cells in the wound granulation tissue， and the number of new blood vessels decreased. Compared with model group， above indexes of PTE low-dose and high-dose groups were improved significantly （P＜0.05）， and the pathological injury of granulation tissue in wound was improved. PP2 significantly reversed the improvement effects of PTE on the above indexes （P＜0.05）. CONCLUSIONS PTE can promote the wound healing of diabetic skin ulcer model rats， the mechanism of which may be related to activating SRC/MEK/ERK signaling pathway.
