Establishment of HPLC fingerprints for Ardisia crenata，Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components
- VernacularTitle:朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定
- Author:
Yun CHEN
1
;
Hui SHI
1
,
2
;
Tingting FENG
1
,
2
;
Liyan ZHANG
1
;
Xiu DONG
3
;
Jinhe ZHANG
1
;
Bei HUANG
4
;
Ying ZHOU
1
Author Information
1. School of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. Research Center for Application and Development of Medicine and Food Dual-use Resource，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
3. Guizhou Sanli Pharmaceutical Co.，Ltd.，Guizhou Anshun 561100，China
4. Inspection Center of Guizhou Provincial Drug Administration，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Ardisia crenata;
Sophora tonkinensis;
couplet medicines;
fingerprint;
content determination;
high-performance
- From:
China Pharmacy
2023;34(16):1949-1954
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the fingerprints of Ardisia crenata， Sophora tonkinensis and their couplet medicines， and to determine the contents of five components in them. METHODS Using water as solvent， single lyophilized powder of A. crenata and S. tonkinensis and combined lyophilized powder of their couplet medicines were prepared by combining lyophilization technology. The fingerprints of three lyophilized powder samples were established by using high-performance liquid chromatography （HPLC）， and the contents of 5 kinds of components such as gallic acid were determined simultaneously. RESULTS There were 5， 10 and 14 common peaks in the fingerprints for single lyophilized powder of A. crenata and S. tonkinensis and combined lyophilized powder of their couplet medicines； the similarities of them with the control fingerprints were all greater than 0.90. For combined lyophilized powder of couplet medicines， peak 3 Δ 基金项目 国家重点研发计划项目（No.2018YFC1708100）；贵 州省科技计划项目（No.黔科合基础-ZK〔2022〕一般483，No.黔科合成 was identified as gallic acid， peak 4 as matrine， peak 6 as 果〔2021〕一般137）；贵州省教育厅高等学校科学研究项目（青年项目） oxymatrine， peak 8 as bergenin， and peak 14 as trifolirhizin. In single lyophilized powder of A. crenata， the average contents of gallic acid and bergenin were 0.499 3 and 4.962 6 mg/g， respectively. In single lyophilized powder of S.tonkinensis， the average contents of matrine， oxymatrine and trifolirhizin were 3.046 0， 2.336 6 and 0.278 6 mg/g， respectively. In combined lyophilized powder of couplet medicines， the average contents of gallic acid， matrine， oxymatrine， bergenin and trifolirhizin were 0.560 6， 2.548 7， 1.382 2， 5.960 7 and 0.279 1 mg/g， respectively. The transfer rates were 8.87%-513.19%. CONCLUSIONS The established fingerprint and content determination methods are stable and feasible， and can be used for the quality control of A. crenata and S. tonkinensis and their couplet medicines. The average contents of matrine and oxymatrine in combined lyophilized powder of A. crenata-S. tonkinensis couplet medicines are decreased.
