Optimization of pretreatment methods for N-nitrosamine compounds in ready-to-eat aquatic products
10.19485/j.cnki.issn2096-5087.2023.08.018
- Author:
CHEN Jing
;
WANG Liyuan
;
HU Zhengyan
;
TANG Jun
;
WU Pinggu
- Publication Type:Journal Article
- Keywords:
N-nitrosamine compound;
ready-to-eat aquatic product;
steam distillation extraction;
liquid-liquid microextraction;
gas chromatography-tandem mass spectrometry
- From:
Journal of Preventive Medicine
2023;35(8):726-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the pretreatment method of N-nitrosamine compounds in ready-to-eat aquatic products.
Methods:Market-sold ready-to-eat aquatic products were collected, homogenized and distilled by steam. The samples were extracted for 10 minutes using dispersive liquid-liquid microextraction (DLLME) with ethanol, trichloromethane and sodium chloride (3.0 g). After centrifugation, the organic phase in the lower layer was collected and subjected to gas chromatography-tandem mass spectrometry (GC-MS/MS). The six common N-nitrosamine compounds were determined in ready-to-eat aquatic products using multiple reaction monitoring mode (MRM) and quantified by the internal standard method.
Results:The optimized method exhibited a good linear relationship at concentrations of 10.0 to 500 μg/L for determination of 6 N-nitrosamine compounds (correlation coefficient of greater than 0.999), with 0.05 to 0.60 μg/kg limit of detection, 0.15 to 1.60 μg/kg limit of quantitation, mean spiked recovery rates of 71.8% to 108.9%, and relative standard deviations of 1.4% to 8.6%. N-Nitrosodimethylamine showed the highest detection rate in 20 market-sold ready-to-eat aquatic products (90%), and the detection rates of N-Nitrosopyrrolidine, N-Nitrosodiethylamine and N-dibutylnitrosamine were 15%, 10% and 10%, respectively.
Conclusion:Steam distillation combined with DLLME may optimize the pretreatment method of N-nitrosamine compounds in ready-to-eat aquatic products and meet the measurement requirements.
- Full text:即食水产制品N-亚硝胺类化合物检测的样品处理方法优化研究1.pdf
