Proteomic Research on Characteristic Processing Materials of Porcine Cardiac Blood and Porcine Blood from Menghe Medical School and Their Effect on Anti-microglial Inflammatory Damage of Salviae Miltiorrhizae Radix et Rhizoma
10.13422/j.cnki.syfjx.20230747
- VernacularTitle:孟河医派特色炮制辅料猪心血和猪血的蛋白质组学分析及其对丹参抗小胶质细胞炎症损伤的影响
- Author:
Nan LIU
1
;
Yuanpei LIAN
1
;
Chunyan YIN
1
;
Jiali CAI
1
;
Dijun WANG
1
;
Li ZHU
1
;
Chanming LIU
1
;
Wei HUANG
1
;
Xiaojing YAN
1
Author Information
1. Changzhou Key Laboratory of Human Use Experience Research & Transformation of Menghe Medical School，Changzhou Hospital of Traditional Chinese Medicine， Nanjing University of Chinese Medicine，Changzhou 213003，China
- Publication Type:Journal Article
- Keywords:
Menghe medical school;
characteristic processing materials;
porcine cardiac blood;
porcine blood;
liquid chromatography-mass spectrometry;
proteomics;
BV2 cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(18):137-145
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveProteomics was used to investigate the protein differences between porcine cardiac blood（PCB） and porcine blood（PB） from Menghe medical school and to compare the effects of both on the microglial inflammation of Salviae Miltiorrhizae Radix et Rhizoma（DS）. MethodNanoliquid chromatography-quadrupole orbitrap mass spectrometry（nLC-MS/MS） and bioinformatics were utilized to compare the proteomic differences of PCB and PB in simulated gastrointestinal digestion. Furthermore， Western blot was used to verify the contents of some shared proteins and differential proteins identified in PCB and PB. In addition， BV2 neuroinflammation model constructed by corticosterone（CORT） and lipopolysaccharide（LPS） was applied to detect the intervention effects of PCB and PB on the levels of tumor necrosis factor（TNF）-α and interleukin（IL）-6 in BV2 inflammatory cells of DS. ResultA total of 69 common proteins and 68 differential proteins were identified in PCB and PB， among which the common proteins included transferrin（Tf） with brain-targeting effect， and the differential proteins in the two were 41 and 27， respectively. Western blot validation showed that the difference in the content of the same protein Tf between PCB and PB was not statistically significant， while the difference in the contents of the specific proteins of creatine kinase M and heart-type fatty acid binding protein（H-FABP） were statistically significant（P<0.05）. Moreover， in vitro experimental studies revealed that compared with the same concentration of DS group， in addition to the 100 mg·L-1 PB-DS group， PCB-DS and PB-DS groups could significantly inhibit the levels of TNF-α and IL-6 in BV2 inflammatory cells（P<0.05， P<0.01）， and PCB-DS group had more significant anti-inflammatory effect than PB-DS group with the same concentration（P<0.05， P<0.01）. ConclusionBoth of PCB and PB can enhance the inhibitory effect of DS on the release of inflammatory factors， thus playing a neuroprotective role， and PCB promotes DS inhibition more significantly， which may be due to the existence of the two involved in energy metabolism-related differential proteins， which can lay a foundation for revealing the scientific connotation of the processing of PCB-DS and PB-DS.
