Effect of Reduning Injection， Shenfu Injection Combined with Gefitinib on Proliferation， Stemness Characteristics and Metabolism of Non-small Cell Lung Cancer Cells
10.13422/j.cnki.syfjx.20222421
- VernacularTitle:热毒宁注射液、参附注射液联合吉非替尼对非小细胞肺癌细胞增殖、干性特征及代谢的影响
- Author:
Zhenzhen XIAO
1
;
Yanjuan ZHU
1
;
Yaya YU
1
;
Lina DING
2
;
Changju MA
1
;
Chenjing LEI
2
;
Yihong LIU
1
;
Xuesong CHANG
1
;
Yadong CHEN
1
;
Haibo ZHANG
1
Author Information
1. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine （Guangdong Provincial Hospital of Chinese Medicine）， Guangzhou 510120， China
2. The Second Clinical College of Guangzhou University of Chinese Medicine，Guangzhou 510120，China
- Publication Type:Journal Article
- Keywords:
Reduning injection;
Shenfu injection;
gefitinib;
non-small cell lung cancer （NSCLC）;
stemness characteristics;
metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(18):45-53
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare and observe the effect of Reduning injection （mainly clearing heat）， Shenfu injection （mainly warming Yang） combined with gefitinib on the proliferation， apoptosis， stemness characteristics and metabolism of lung cancer cells. MethodDifferent non-small cell lung cancer （NSCLC） cell lines were selected and intervened with gefitinib （5， 10， 20 μmol·L-1）， Reduning injection （0.6%， 0.9%）， Shenfu injection （0.6%， 0.9%）， gefitinib combined with Reduning injection， and gefitinib combined with Shenfu injection. Cell proliferation in each group was detected by cell counting kit-8 （CCK-8） assay， and cell apoptosis was detected by flow cytometry. The mRNA and protein expressions of lung cancer stem cell markers sex determining region Y-box 2 （Sox2） and aldehyde dehydrogenase family 1 member A1 （ALDH1A1） were determind by real-time quantitative polymerase chain reaction （Real-time PCR） and Western blot， respectively. The redox ratio of lung cancer cells was observed by femtosecond label-free imaging （FLI） and energy metabolism instrument was used to determine the glycolysis level in cells. ResultCompared with the blank group， Reduning injection reduced the survival rate of lung cancer cells （P<0.05）， increased the apoptosis rate （P<0.05）， down-regulated the mRNA and protein expressions of Sox2 and ALDH1A1 （P<0.05）， and up-regulated the redox ratio of cells （P<0.05）， while Shenfu injection exerted no remarkable effect on the above indexes. In addition， compared with gefitinib alone， Reduning injection combined with gefitinib inhibited the survival rate of lung cancer cells （P<0.05）， promoted the cell apoptosis （P<0.05）， down-regulated the mRNA and protein expressions of Sox2 and ALDH1A1 （P<0.05）， up-regulated the redox ratio of cells （P<0.05）， and lowered the proton efflux rate of glycolysis （P<0.05）， while Shenfu injection combined with gefitinib failed to affect these indexes of lung cancer cells significantly. ConclusionReduning injection may inhibit stemness characteristics of tumor cells by regulating their metabolism to enhance the proliferation-inhibiting and pro-apoptotic effects of gefitinib on lung cancer cells， while Shenfu injection had no significant enhancing effect on gefitinib. This indicates that epidermal growth factor receptor tyrosine kinase inhibitors （EGFR-TKIs） should be used in combination with heat-clearing Chinese medicines.
