Effect of stem cell factor on the angiogenic ability of cocultured DPSCs and HUVECs
10.12016/j.issn.2096-1456.2023.10.004
- Author:
JI Hongjiao
1
;
XU Lili
2
;
DING Wentong
1
;
LI Peihui
1
;
WANG Yanjiu
1
;
PAN Shuang
1
Author Information
1. The First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University
2. Second Clinical Division, Peking University Hospital of Stomatology
- Publication Type:Journal Article
- Keywords:
dental pulp regeneration / angiogenesis / stem cell factor / growth factor / dental pulp stem cells / humman umbilical vein endothelial cells / coculture
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2023;31(10):712-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of stem cell factor (SCF) on the angiogenic ability of cocultured dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs).
Methods :This study has been reviewed and approved by the Ethics Committee. The experiment was split into the HUVECs, SCF+HUVECs, DPSCs+HUVECs, and SCF+DPSCs+HUVECs groups. A mixture of SCF and culture medium was used to prepare a mixed culture medium with an SCF concentration of 100 ng/mL. In vitro coculture of DPSCs and HUVECs was performed at a 1∶5 ratio. CCK-8 proliferation assay was used to observe the proliferative capacity of cells in each group on days 1, 3, 5, and 7. Wound healing and Transwell migration assays were used to detect the effect of SCF on cell migration under either direct or indirect coculture conditions, respectively. In vitro angiogenesis experiments were performed to detect the angiogenic capacity of the cells in each group. The vascular endothelial growth factor A (VEGFA) concentration in the cell culture supernatant was detected using ELISAs, and the protein expression levels of CD31, CD34, and VEGFA were detected using Western blot analysis.
Results : Wound healing and Transwell migration experiments showed that SCF significantly promoted the migration of cocultured DPSCs and HUVECs (P<0.05). The in vitro angiogenesis experiment showed that the number of branches and the total length of branches of tubular structures in the SCF+DPSCs+HUVECs group were significantly greater than those of the other groups (P<0.05), and the expression levels of the vascular-related proteins CD31, CD34, and VEGFA in this group were greater (P<0.01).
Conclusion : SCF can enhance the migration and in vitro angiogenesis of cocultured DPSCs and HUVECs.
- Full text:干细胞因子促进共培养DPSCs与HUVECs的成血管能力.pdf
