Comparative Evaluation of Mouse Bone Marrow Mesenchymal  Stromal Cells Characteristics Cultured in Two Different  Supplemented Media

Kwan Liang Lye; Norshariza Nordin; Sharmili Vidyadaran; Niu Jin Tan; Rohayu Izanwati Mohd Rawi; Karuppiah Thilakavathy

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Comparative Evaluation of Mouse Bone Marrow Mesenchymal Stromal Cells Characteristics Cultured in Two Different Supplemented Media

Author: Kwan Liang Lye ¹ ; Norshariza Nordin ^{1
,

2} ; Sharmili Vidyadaran ^{3
,

4} ; Niu Jin Tan ¹ ; Rohayu Izanwati Mohd Rawi ^{1
,

5} ; Karuppiah Thilakavathy ^{1
,

2}
Author Information

1. Medical Genetics Unit, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia&
2. Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3. Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia&
4. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
5. Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Kampus Gong Badak, 21300 Kuala Nerus, Terengganu, Malaysia
Publication Type:Journal Article
Keywords: Mesenchymal stromal cell, Characterization, Mouse bone marrow
From:Malaysian Journal of Medicine and Health Sciences 2022;18(No.1):222-233
CountryMalaysia
Language:English
Abstract: Introduction: Preclinical studies on mesenchymal stromal cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. In recent years, conflicting data have been reported regarding various aspects of their characteristics, development and differentiation potential, which may be due to arrange of factors. Among the factors worth investigating is the culture medium formulation. Methods: Here we have made a comparative characterization of mouse bone marrow mesenchymal stromal cells (mBM-MSC) that were cultured using two common supplements, MesenCult™ Stimulatory Supplement (MSS) and fetal bovine serum (FBS), under the same experimental conditions at different passages. Clonogenic potential, cumulative population doubling level (CPDL), population doubling time (PDT), immunophenotyping, differentiation, immunosuppression potentials and chromosome analysis of early and late passages mBM-MSC were assessed. Results: Our findings showed that the CPDL, immunophenotype and immunosuppression potential of mBM-MSC were similar. However, variations were seen in their clonogenicity, population doubling time and differentiation efficacy whereby all of these were enhanced in DMSS. These observations suggest that their genetic make-up may be affected by both supplements upon prolonged culture. Interestingly, this conjecture was supported when chromosomal analysis revealed genetic instability of mBM-MSCs cultured in both supplements. Conclusion: In conclusion, culture medium formulation was shown to cause variations and spontaneous transformation in mBM-MSCs raising concerns on the usage of late passages mBMMSCs in fundamental and preclinical downstream experiments.