Fabrication, Physicochemical and Rheological Characterisation  of a Drug-therapeutic Oils (Doxycycline Hyclate-Nigella  sativa-Eugenol) Complex Emulsion Stabilised by Lecithin and  Hydroxypropyl Methylcellulose Intended for Delivery Into  Periodontal Pocket

Farahidah Mohamed; Nurhani Emira Hamidon; Anugerah Budipratama Adina; Yunita Dewi Ardini; Affendi Mohd Shafri

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Fabrication, Physicochemical and Rheological Characterisation of a Drug-therapeutic Oils (Doxycycline Hyclate-Nigella sativa-Eugenol) Complex Emulsion Stabilised by Lecithin and Hydroxypropyl Methylcellulose Intended for Delivery Into Periodontal Pocket

Author: Farahidah Mohamed ¹ ; Nurhani Emira Hamidon ² ; Anugerah Budipratama Adina ³ ; Yunita Dewi Ardini ⁴ ; Affendi Mohd Shafri ²
Author Information

1. Pharmaceutical Technology Department, Kulliyyah of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
2. Biomedical Science Department, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
3. Pharmaceutics Unit, School of Pharmacy, Management and Science University, 40100 Shah Alam Selangor Darul Ehsan, Malaysia
4. Paediatric Dentistry and Dental Public Health Department, Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
Publication Type:Journal Article
Keywords: Lecithin, Doxycycline, Nigella sativa, Eugenol, Chronic periodontitis
From:Malaysian Journal of Medicine and Health Sciences 2022;18(No.1):20-28
CountryMalaysia
Language:English
Abstract: Introduction: Earlier attempts to stabilise an emulsion, intended for chronic periodontitis treatment which composed of doxycycline hyclate (DH), Nigella sativa oil (NSO), eugenol and several combinations of surfactants failed. To solve the issue, we investigated the ability of lecithin alone and its combination with hydroxypropyl methylcellulose (HPMC) to stabilise the emulsion. Method: Compatibility between DH and other ingredients was first investigated using DSC and ATR-IR. The emulsion was characterised, firstly by preparing three phases: doxycycline/preservatives with or without HPMC (varying quantities), NSO/eugenol and lecithin/surfactants as aqueous, oil and emulsifier phases, respectively. The phases were added and emulsified sequentially at 7000 rpm (10 min) with an overhead stirrer and then at 3000 rpm (15min) using a high-shear mixer. DH assay was performed using validated HPLC method. Results: All ingredients were found to be compatible with doxycycline based on DSC, ATR-IR and supported by acceptable recovery (98.2±2.2 %) of DH from the emulsion. Stable emulsions were produced with particle size of 198.6±8.2 to 279.3±10.7 nm and zeta potential of -48.2±0.4 to -64.0±3.9 mV. The emulsions showed high viscosity (~200 Pa.s) at zero shear rate and exhibited shear-thinning flow upon increased in shear stress yielding viscosity of ~3 Pa.s at 100 s-1 indicating pseudoplastic behaviour suitable for pre-filled syringe packaging intended for delivery into periodontal pocket. Conclusion: Lecithin is an excellent emulsifier that can also impart pseudoplasticity for a complex emulsion constitute of drug and natural oils. This could pave the way for a more complex emulsion formulation fusing contemporary and therapeutic oils
Full text:11.2022my1176.pdf