Effects of Angelica sinensis on the expression of vascular endothelial growth factor after the brain ischemia-reperfusion injury in rats
- VernacularTitle:当归注射液对大鼠脑缺血再灌注损伤后VEGF表达的影响
- Author:
Chanjuan ZHENG
;
Weijing LIAO
;
Wantong YANG
;
Lanqing MENG
- Publication Type:Journal Article
- Keywords:
brain ischemia-reperfusion injury, vascular endothelial growth factor (VEGF), Angelica sinensis injection
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(12):973-974
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of Angelica sinensis on the expression of vascular endothelial growth factor (VEGF) Flt-1, Flk-1 mRNA after the brain ischemia-reperfusion injury in rats.MethodsWistar rats were randomly divided into the group A, group B and normal control group. The group A underwent middle cerebral artery occlusion (MCAO) for 2h by suture, group B underwent MCAO for 2h meanwhile received treatment with Angelica sinensis (5 g/kg). Immunohistochemistry and quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique were used to examine the gene expression of VEGF.ResultsThe result of immunohistry revealed that VEGF in the group A and group B reached its peak at 24 h after reperfusion then declined gradually. The result of RT-PCR manifested that the gene expression of VEGF in the group A increased from 3 h after reperfusion and reached its peak at 6 h; in the group B reached its peak on the 3rd day. The expression of VEGF in the group B was significantly increased than group A at the same time point.ConclusionAngelica sinensis can enhance the expression of Flt-1, Flk-1 after transient interruption of cerebral blood flow in rats.
