Co-culture of Schwann cell with fascia
- VernacularTitle:Schwann细胞与筋膜共同培养的实验研究
- Author:
Chang-xiang YAN
;
Hong WAN
;
Jun-hua LI
;
Chunjiang YU
;
Zhongcheng WANG
- Publication Type:Journal Article
- Keywords:
Schwann cell, fascia, culture in vitro
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(11):660-661
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the method of co-culture of Schwann cell(SCs) with fascia and provide experimental basis for repairing transected nerve.MethodsSCs were co-cultured with fascia.Double staining by anti-BrdU and anti-S-100,S-100 fluorescent staining and anti-BrdU staining were used.ResultsThere were a plenty of SCs around fascia proliferated rapidly and disposed in parallel. SCs could be distinguished from fibroblastic cells by S-100 fluorescent staining and also be staining positive by anti-BrdU antibody,implying their high proliferous ability. Anti-BrdU and anti-S-100 staining showed numerous double staining positive SCs on the fascia: nucleus was stained deep blue while cytoplasm was stained red.ConclusionMany SCs with high proliferous ability were seen on the fascia, which can be used to repair transected nerve.
