Development of long-term cell culture facility in hypothermia and its application in space biology research
- VernacularTitle:细胞低温长期生存系统的研究以及在空间搭载中的应用
- Author:
Qing FANG
;
Mei XU
;
Bo XU
;
Hongyan LI
;
Qing XIANG
;
Zhihua CHEN
;
Xuan LIU
;
Jintian TANG
- Publication Type:Journal Article
- Keywords:
hypothermia, cell culture, spaceflight, culture facility
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(11):649-651
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo develop the facility of mammalian cell culture in hypothermia for long-term, and to lay the base for carrying mammalian cells to outer space and establishing outer space biomedical research stage. MethodsMultiple cell lines were maintained in the spacial designed hypothermia cell culture facility and carried to space by Shenzhou 4 and No.18 recoverable satellite. Cell morphology, cell multiplication and the ability to be monocloned were observed.ResultsCells were successfully maintained in the hypothermia cell culture facility for up to 26 days without obvious changes of cell morphology. The cells could normally grow, multiple and be monocloned after inoculated in 37 ℃ for 8 h.ConclusionA hypothermia cell culture facility was successfully established,which ensure technically mammalian cells to be carried by space craft and further research on space radiation.
