Preliminary study of tumor cells and bio-engineering cells carried by returned satellite
- VernacularTitle:返回式卫星搭载肿瘤细胞和生物工程细胞的初步研究
- Author:
Qing XIANG
;
Mei XU
;
Hong-yan LI
;
Bo XU
;
Qing FANG
;
Xuan LIU
;
Jintian TANG
- Publication Type:Journal Article
- Keywords:
satellite carry, tumor cells, bio-engineering cells, mutation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(11):644-646
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effects on growth characteristics of cells mutated by outer space. Methods5 tumor cell lines and 1 bio-pharmaceutical cell line were carried in the recoverable satellite No.18 and returned after 18 days flying in outer space.The cell living systems were utilized in passive carrying. Some survival cells were monocoloned, and the experimental methods such as cell morphological observation,MTT assay and FACS were used for analysis of cell growth characteristics. ResultsThe survival cell rates of tumor cell lines and bio-pharmaceutical cells were markedly different. Compared with the control group, mutated cells appeared to have multiple cell morphological changes and the cell number of G1 phase was increased markedly (P<0.05). Growth rate in mutated cells were varied without specific discipline. ConclusionEffects of outer space on growth characteristics were complicated and multiplex in mutated cells,which were not all heredity in further passage. The differences of the monoclonal cell lines provide the potential to obtain optimizing cell lines by screen.
