A potential mating-type biomarker to detect pathogenic Ganoderma species

Doris Lau; Lee Weng Wah; Chong Mei Ling; Tee Sue Sean; Jonathan Guyang Ling; Anis Farhan Fatimi Ab Wahab; Farah Diba Abu Bakar

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A potential mating-type biomarker to detect pathogenic Ganoderma species

Author: Doris Lau ^{1
,

2} ; Lee Weng Wah ³ ; Chong Mei Ling ³ ; Tee Sue Sean ³ ; Jonathan Guyang Ling ⁴ ; Anis Farhan Fatimi Ab Wahab ⁵ ; Farah Diba Abu Bakar ⁴
Author Information

1. ACGT Sdn Bhd, Lot L3-I-1, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia. &
2. Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia.
3. ACGT Sdn Bhd, Lot L3-I-1, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
4. Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia.
5. FGV Innovation Centre (Biotechnology), PT. 23417 Lengkuk Teknologi, 71760 Bandar Enstek, Negeri Sembilan D.K., Malaysia.
Publication Type:Other Types
Keywords: Pheromone receptor; Ganoderma boninense; Basal stem rot disease; Oil palm
MeSH: Genes, Mating Type, Fungal; Ganoderma
From:Malaysian Journal of Microbiology 2022;18(3):331-337
CountryMalaysia
Language:English
Abstract: Aims:The basal stem rot disease in oil palm is caused by the pathogenic Ganoderma boninense, which is infectious after mating and forming dikaryotic hyphae. This study was aimed to generate a mating-type biomarker for the detection of pathogenic Ganoderma species.
Methodology and results:Mating-type region of Ganoderma was amplified using polymerase chain reaction (PCR) and primers flanking the mating-type region of other basidiomycetes. Amplified fragments were sequenced and were identified as the Ganoderma pheromone receptor gene of matB locus called the gprb2 gene. Using this biomarker, the pheromone receptor gene was detected in a total of 107 pathogenic Ganoderma spp. while the gene was not detected in the non-pathogenic Ganoderma lucidum. Phylogenetic tree analyses of the gene fragment encoding the partial amino acid sequence of gprb2 showed clades of close evolutionary relationship among the 107 pathogenic Ganoderma spp. Phylogenetic analyses using deduced amino acid sequences of the Ganoderma pheromone receptor b2 gene, gprb2 with homologous pheromone receptors of other basidiomycetous fungi revealed high conservation of this pheromone receptor within their respective taxonomy.
Conclusion, significance and impact of study:A potential mating-type biomarker was successfully identified that could detect pathogenic Ganoderma spp. The research findings will be helpful in oil palm screening to detect pathogenic Ganoderma spp. and gain further insight into the role of the mating-type loci of Ganoderma towards its pathogenesis in causing the basal stem rot disease of oil palm.
Full text:20.2022my0035.pdf