Effects of glucose as carbon catabolite repressor on alpha-amylase and glucoamylase production in Indonesian indigenous fungi

Ika Octariyani Safitri; Dian Anggraini Suroto; Jaka Widada; Sardjono; Muhammad Nur Cahyanto

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Effects of glucose as carbon catabolite repressor on alpha-amylase and glucoamylase production in Indonesian indigenous fungi

Author: Ika Octariyani Safitri ¹ ; Dian Anggraini Suroto ¹ ; Jaka Widada ² ; Sardjono ¹ ; Muhammad Nur Cahyanto ¹
Author Information

1. Gadjah Mada University, Faculty of Agricultural Technology, Department of Food and Agricultural Product Technology, Jl. Flora, Bulaksumur, 55281 Yogyakarta, Indonesia.
2. Gadjah Mada University, Faculty of Agriculture, Department of Agricultural Microbiology, Jl. Flora, Bulaksumur, 55281 Yogyakarta, Indonesia.
Publication Type:Journal Article
Keywords: Alpha-amylase; Glucoamylase; Indigenous fungi; Glucose; Repression
MeSH: Glucosidases; alpha-Amylases; Glucan 1,4-alpha-Glucosidase
From:Malaysian Journal of Microbiology 2022;18(3):322-330
CountryMalaysia
Language:English
Abstract: Aims:The study aimed to investigate the effect of glucose on alpha-amylase and glucoamylase production in some Indonesian indigenous fungi.
Methodology and results:Fungi were screened for their ability to produce alpha-amylase and glucoamylase in the presence of glucose. The strains were grown in a medium containing starch and glucose as carbon sources with glucose concentrations varying from 0 to 5% for four days, and the alpha-amylase and glucoamylase were analyzed at the end of the growth period. Most strains showed repression on the amylases production when glucose was added to the medium. However, some strains showed no repression on amylases production when glucose was supplemented to the medium. The addition of glucose repressed glucoamylase production, but no repression on alpha-amylase was noted for strain KKB4, vice versa, there was repression on alpha-amylase production but no repression on glucoamylase production for strain FIG1. Strains FNCC 6151 and MLT1J1 showed no repression on both alpha-amylase and glucoamylase production when glucose was added to the medium up to 5%. The occurrence of repression in the production of alpha-amylase and glucoamylase was strain-specific.
Conclusion, significance and impact of study:Out of the nine indigenous fungi strains examined, strains FNCC 6151 and MLT1J1 showed no repression on both alpha-amylase and glucoamylase production when glucose was added to the medium up to 5%. Those two strains have the potential to be improved further to produce both alpha-amylase and glucoamylase.
Full text:20.2022my0034.pdf