The cagA, cagE, vacA, dupA and iceA1 genes of Helicobacter pylori in Sudanese gastritis patients: Distribution and relationship with clinical outcomes and histological alterations

Maram M Elnosh; Yousif Fadalla Hamedelnil; Wafa A Elshareef; Aliaa Yahia Abugrain; Esraa Hassan Osman; Aalaa Mahgoub Albasha; Khawla Hassan Aseel; Fatima Youssif Ali; Wafaa Mohammed Abdalla; Ahmed Bakheet Abdalla; Tagwa Salah Ahmed Mohammed Ali; Hisham N Altayb

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The cagA, cagE, vacA, dupA and iceA1 genes of Helicobacter pylori in Sudanese gastritis patients: Distribution and relationship with clinical outcomes and histological alterations

Author: Maram M. Elnosh ¹ ; Yousif Fadalla Hamedelnil ¹ ; Wafa A. Elshareef ² ; Aliaa Yahia Abugrain ³ ; Esraa Hassan Osman ¹ ; Aalaa Mahgoub Albasha ¹ ; Khawla Hassan Aseel ¹ ; Fatima Youssif Ali ¹ ; Wafaa Mohammed Abdalla ¹ ; Ahmed Bakheet AbdAlla ⁴ ; Tagwa Salah Ahmed Mohammed Ali ⁵ ; Hisham N. Altayb ⁶
Author Information

1. Microbiology Department, Sudan University of Science and Technology, College of Medical Laboratory Science, Khartoum, Sudan.
2. Histopathology Department, The National Public Health Laboratory, Khartoum, Sudan.
3. Histopathology Department, Sudan University of Science and Technology, College of Medical Laboratory Science, Khartoum, Sudan.
4. Parasitology and Medical Entomology Department, Sudan University of Science and Technology, College of Medical Laboratory Science, Khartoum, Sudan.
5. Department of Clinical Laboratory Science, College of Applied Medical Science, JOUF University, Al_Jouf, Saudi Arabia.
6. Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21452, Saudi Arabia.
Publication Type:Journal Article
Keywords: Helicobacter pylori; Peptic ulcer; Virulence genes; PCR; Sudan
MeSH: Helicobacter pylori; Gastritis
From:Malaysian Journal of Microbiology 2022;18(3):261-270
CountryMalaysia
Language:English
Abstract: Aims:Helicobacter pylori is a gastrointestinal bacterium that causes peptic ulcers and stomach cancer in nearly half of the world’s population. Many virulence factors influence the outcome of H. pylori related disorders. The purpose of this study was to see if there was a relationship between H. pylori virulence factors and histological and endoscopic findings in stomach biopsy specimens from Sudanese gastritis patients.
Methodology and results:In the period between March 2018 and January 2020, a total of 290 gastric biopsies were taken from patients in Khartoum State hospitals. Histopathology and polymerase chain reaction (PCR) assays were performed on all specimens. Histological investigation revealed H. pylori in 103/290 (35.5%) samples, while PCR revealed H. pylori 16S rRNA positivity in 88/290 (30.3%) samples. Eighty-eight positive PCR specimens were subjected to PCR for genotypic detection of cagA, cagE, vacA, dupA and iceA1 genes. All of strains were vacA positive 100% (88/88) followed by dupA 50.0% (44/88), cagA 40.9% (36/88), cagE gene 38.6% (34/88) and iceA1 gene was detected in only 15.9% (14/88). The vacA s1/m1 68.2% (60/88) was the most prevalent vacA subtype.
Conclusion, significance and impact of study:Helicobacter pylori virulence genes were widespread and diversified in Sudanese gastritis patients. Helicobacter pylori cagA and iceA1 were significantly in association with gastric mucosa inflammation degree, whereas the dupA gene was found to be associated with the clinical outcomes.
Full text:20.2022my0027.pdf