Multiplication and differentiation into endothelial cells of bone marrow stromal cells of rats in vitro
- VernacularTitle:大鼠骨髓基质细胞体外诱导分化为内皮细胞实验研究
- Author:
Chun-xue WANG
;
Hong WAN
;
Jun-hua LI
;
Yongjun WANG
;
Shujing MAO
;
Liping LIU
;
Jing ZHAI
- Publication Type:Journal Article
- Keywords:
recombinant human GM-CSF(rhGM-CSF), bone marrow stromal cells(BMSC), endothelial cell, von Willebrand factor(vWF), factor Ⅷ related antigen (FⅧ-R Ag)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(8):458-460
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the feasibility of bone marrow stromal cells (BMSC) of rats differentiating into endothelial cells.MethodsBone marrow cells were obtained by an aseptic technique. Afterwards, the obtained cells were divided into two groups: cells in test group were propagated in 1640 medium with recombinant human GM-CSF (rhGM-CSF) (400ng/ml), and that in control group were propagated in medium with 1640 medium only. The differentiated cells were detected by specific immunology marker by immunocytochemistry and immunofluorescence at day 8.ResultsThe differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy. Cells of the test groups demonstrated specific characters by immunocytochemistry stain.ConclusionBone marrow stromal cells can multiply vigorously and differentiate into cells with endothelial cells characters in the medium with high concentration rhGM-CSF.
