Effects of polyphyllin Ⅵ on the proliferation and apoptosis of glioma cells and potential mechanism
- VernacularTitle:重楼皂苷Ⅵ对脑胶质瘤细胞增殖和凋亡的影响及潜在机制
- Author:
Fang WANG
1
;
Yunyang LU
2
;
Weiwei LI
3
;
Minna YAO
3
Author Information
1. School of Medical Technology，Shaanxi Energy Institute，Shaanxi Xianyang 712000，China
2. Dept. of Traditional Chinese Medicine and Natural Pharmacology，Air Force Medical University，Xi’an 710032，China
3. Dept. of Pharmacy，Xijing Hospital，Air Force Medical University，Xi’an 710032，China
- Publication Type:Journal Article
- Keywords:
polyphyllin Ⅵ;
human glioma cell;
proliferation;
apoptosis;
Fas/FasL death receptor pathway;
Akt/GSK-3β
- From:
China Pharmacy
2023;34(14):1686-1690
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of polyphyllin Ⅵ（PPⅥ） on the proliferation and apoptosis of glioma cells and potential mechanism. METHODS Using human glioma LN229 cells as objects， MTT assay was used to detect the survival rate after treated with different concentrations of PPⅥ [0 （control group）， 1， 2， 4， 8， 16， 32， 64 μmol/L] for different time （24， 48， 72 h）. The clone formation experiments were adopted to detect the number of cell clones and clone formation rate after being treated with different concentrations of PPⅥ [0 （control group）， 2， 4， 8 μmol/L] for 14 days. The flow cytometry and Western blot assay were used to detect the apoptotic rate of cells， the expressions of apoptosis-related protein [B cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， cleaved caspase-3]， and the expressions of related proteins of Fas/Fas ligand （FasL） death receptor pathway and protein kinase B （Akt）/glycogen synthesis kinase-3β （GSK-3β） pathway after being treated with different concentrations of PPⅥ [0（control group）， 4， 8 μmol/L] for 24 h. RESULTS Compared with the control group， the survival rate of cells， the number of clones and clone formation rate， the protein expression of Bcl-2， and the phosphorylation levels of Akt and GSK-3β protein were decreased significantly in different concentration groups of PPⅥ （P＜0.05 or P＜0.01）. The apoptotic rate， the protein expressions of Bax， cleaved caspase-3， Fas， FasL and cleaved caspase-8 were increased significantly （P＜0.05 or P＜ 0.01）. CONCLUSIONS PPⅥ can inhibit the proliferation and induce the apoptosis of human glioma LN229 cells， which may be related to the activation of the Fas/FasL death receptor pathway and the inhibition of the Akt/GSK-3β pathway.
