Study on the intestinal absorption characteristics of saikosaponins
- VernacularTitle:柴胡皂苷类成分的肠吸收特性研究
- Author:
Yazhi WANG
1
;
Qiyi WANG
1
;
Wenzhong FENG
1
;
Shuangshuang CHEN
1
;
Xinguang SUN
2
;
Lijuan ZHOU
2
;
Yan ZHANG
1
;
Jianyong ZHANG
1
;
Cancan DUAN
3
Author Information
1. College of Pharmacy，Zunyi Medical University，Guizhou Zunyi 563000，China
2. Beijing Zhendong Guangming Pharmaceutical Research Institute Co.，Ltd.，Beijing 100085，China
3. Key Lab of Basic Pharmacology of Ministry of Education & Joint International Research Laboratory of Ethnomedicine of Ministry of Education，Zunyi Medical University，Guizhou Zunyi 563000，China
- Publication Type:Journal Article
- Keywords:
Bupleurum chinense;
saikosaponins;
everted intestinal sac experiment;
accumulative absorption amount
- From:
China Pharmacy
2023;34(14):1681-1685
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the intestinal absorption characteristics of saikosaponins. METHODS Based on everted intestinal sac model， using accumulative absorption amount （Q） and absorption rate constant （Ka） as indexes， UHPLC-MS/MS technique as a method， the absorption of saikosaponin A， B2， C， D and F from total saponins of Bupleurum chinense （8 g/mL， by crude drug） in the duodenum， jejunum and ileum was detected. RESULTS The correlation coefficients （r） of the regression equations for the absorption of saikosaponins A， B2， C and F in the duodenum， jejunum and ileum were all higher than 0.95， while the r of saikosaponin D in the above intestinal segments was lower than 0.95； compared with the absorption of the same composition in the duodenum， the Q and Ka of saikosaponin A and C circulating in jejunum and ileum for 120 min， as well as the Q and Ka of saikosaponin F circulating in the ileum for 120 min were significantly decreased （P＜0.05）. CONCLUSIONS Saikosaponin A and the other 4 saikosaponins are all absorbed in the duodenum， jejunum and ileum； among them， saikosaponin A， B2， C and F are linearly absorbed， which conforms to the zero-order absorption characteristics， but saikosaponin D shows non- linear absorption.
