Extraction of effective components of Psoralea corylifolia and efficacy evaluation in the treatment of vitiligo
- VernacularTitle:补骨脂有效成分的提取及治疗白癜风药效评价
- Author:
Xuemei YU
1
;
Zhenghua WU
1
;
Guorong FAN
1
Author Information
1. Dept. of Clinical Pharmacy，Shanghai General Hospital，Shanghai Jiao Tong University School of Medicine，Shanghai 200080，China
- Publication Type:Journal Article
- Keywords:
Psoralea corylifolia;
effective component;
extraction process;
analytic hierarchy process;
Plackett-Burman design
- From:
China Pharmacy
2023;34(14):1676-1680
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To extract the effective components of Psoralea corylifolia and evaluate its efficacy in the treatment of vitiligo. METHODS The concentrations of psoralen， isopsoralen， neobavaisoflavone， corylin， psoralidin， corylifolinin， and bakuchiol in P. corylifolia extract were determined by ultra-performance liquid chromatography. Based on the analytic hierarchy process （AHP） and Plackett-Burman design， with the concentrations of the 7 components as evaluation indexes and the crushing degree， ethanol concentration， and soaking time as factors， the extraction process of P. corylifolia was optimized by Box-Behnken response surface methodology and the validation test was conducted. Zebrafish were divided into blank control group， positive control group （8-methoxypsoralen， 10.8 μg/mL）， and low-， medium-， and high-concentration groups of P. corylifolia extract （500， 1 000， 2 000 μg/mL）， with 6 fish in each group. The effects of P. corylifolia extract on the melanin production of zebrafish were studied by density analysis. RESULTS The best extraction process was P. corylifolia powder over 60 meshes and soaked in 80% ethanol for 72 hours. The average comprehensive score of three validation experiments was 98.27， with an RSD of 1.36%， and the relative error was 1.02% compared with the predicted value of the fitting equation （97.28）. Compared with the blank control group， the melanin pigmentation of zebrafish in the low-， medium-， and high-concentration groups of P. corylifolia extract was significantly increased （P＜0.01）. CONCLUSIONS The optimized extraction process of P. corylifolia is reasonable and feasible， and the obtained P. corylifolia extract can significantly promote the production of melanin in zebrafish.
