Determination of the antiangiogenic activity of Telescopium telescopium (Horn snail) extract using in ovo chorioallantoic membrane (CAM) assay.
https://doi.org/10.47895/amp.v52i4.379
- Author:
Milany Anne M. Luay
1
;
Mary France R. Gonzaga
1
,
2
;
Sharmaine Kae D. Po
1
;
Erna C. Arollado
1
,
2
Author Information
1. Department of Pharmacy, College of Pharmacy, University of the Philippines Manila
2. Institute of Pharmaceutical Sciences, National Institutes of Health, University of the Philippines Manila
- Publication Type:Journal Article
- Keywords:
Antiangiogenic;
Anticancer;
Chorioallantoic;
Membrane Assay;
Mollusk;
Telescopium Telescopium;
Horn Snail
- MeSH:
Chorioallantoic Membrane;
Gastropoda
- From:
Acta Medica Philippina
2018;52(4):366-373
- CountryPhilippines
- Language:English
-
Abstract:
Objective:To determine the antiangiogenic activity of Telescopium telescopium (Horn snail) extract using in ovo chorioallantoic membrane (CAM) assay.
Methods:Methanolic extract of Telescopium telescopium was subjected to modified Kupchan partitioning. Four treatment groups – negative control, positive control (quercetin), test samples, and blanks – were used for the in ovo chorioallantoic membrane (CAM) assay. ImageJ software was used to measure average vessel diameter (DV) and total length (LT) to determine the degree of vascularization, percent inhibition, and antiangiogenic activity. Biochemical screening was done for the crude extract and the fraction with the highest percent inhibition.
- Full text:acta 3.pdf
