Apoptosis and expression of iNOS after spinal cord injury in rats
- VernacularTitle:脊髓损伤后细胞凋亡及iNOS的表达
- Author:
Qiang AO
;
Decheng LV
;
Changming JIANG
;
Fenglin GUAN
;
Yuan CI
- Publication Type:Journal Article
- Keywords:
spinal cord injuries, apoptosis, inducible nitric oxide synthase(iNOS), gene expression
- From:
Chinese Journal of Rehabilitation Theory and Practice
2003;9(1):38-41
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the characteristic of apoptosis and the expression of inducible nitric oxide synthase(iNOS),as well as relation between them after spinal cord injury (SCI) in rats.Methods84 adult SD rats were randomly divided into three groups,and made into mild,moderate and severe acute SCI models.The rats were killed at 4h,8h,1d,3d,7d,14d and 21d after surgery.The histopathology changes in spinal cord tissue were observed with HE staining microscopic examination.The expression of iNOS was determined by immunohistochemistry and the apoptosis was labeled by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).ResultsThe histopathology changes in spinal cord tissue deteriorated with increasing in injury degree.A little iNOS immunoreactivity (iNOS-IR) was detected in nerve cells,gliocytes,ependymocytes and blood vessel endotheliocytes of normal and vertebra shelf operated controls,increasing at 8 h, and in flood tide in 7 d. The expression of Bax and the rates of TUNEL positive cells were similar with that of iNOS after SCI. There was positive correlation between expression of iNOS and degree of primary SCI ,apoptosis index(r=0.414,P<0.01;r=0.854,P<0.01).Conclusions Expression of iNOS and Bax increase and a large number of TUNEL positive cells present after SCI in rats. There is positive correlation between expression of iNOS and degree of primary SCI,apoptosis index.
