Research on the anti-tumor effect of nordihydroguaiaretic acid targeting MyD88
10.16438/j.0513-4870.2022-1244
- VernacularTitle:去甲二氢愈创木酸靶向MyD88抗肿瘤研究
- Author:
Yue WANG
1
,
2
;
Yi QU
1
,
2
;
Xi-song KE
1
,
2
;
Xue ZHANG
1
,
2
Author Information
1. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Shanghai Frontiers Science Center of TCM Chemical Biology, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
myeloid differentiation primary response protein 88;
colon cancer;
skin cancer;
pancreatic cancer;
NF-κB signaling pathway;
nordihydroguaiaretic acid
- From:
Acta Pharmaceutica Sinica
2023;58(4):946-953
- CountryChina
- Language:Chinese
-
Abstract:
This study mainly explores the role of myeloid differentiation primary response protein 88 (MyD88) in tumorigenesis and development, to identify active compounds targeting MyD88. CRISPR/Cas9 system and xenograft tumor model were used to detect the effect of MyD88 deletion on tumor growth, and the experimental animal ethics review number was PZSHUTCM200828006. Microscale thermophoresis technology (MST) was used to identify compounds directly bind to MyD88 and further detect the impact of candidate small molecules on cell proliferation. Results showed that depletion of MyD88 significantly inhibited xenograft tumor growth of colon cancer, pancreatic cancer and skin cancer and the activity of NF-κB signaling pathway. MST showed that nordihydroguaiaretic acid (NDGA) bound to MyD88, with the binding dissociation constant Kd of 14.61 µmol·L-1. NDGA inhibited NF-κB reporting system activation and phosphorylation of p65, the key factor in NF-κB signal pathway. In addition, the results of colony formation assay showed that NDGA suppressed the proliferation of tumor cells. The above results show that, MyD88 is a potential therapeutic target for colon cancer, pancreatic cancer and skin cancer, NDGA directly binds to MyD88 and inhibits the activity of NF-κB signaling pathway, as well as inhibits the proliferation of pancreatic cancer, skin cancer and colon cancer cells.
