Study on the objectivity and biological mechanism of Psoralea corylifolia Linn.'s 'Great dryness damages the liver'
10.16438/j.0513-4870.2022-1222
- VernacularTitle:补骨脂“大燥伤肝”的客观性及生物学机制研究
- Author:
Ming-liang ZHANG
1
,
2
,
3
;
Xu ZHAO
4
;
Wei-xia LI
5
;
Xiao-yan WANG
5
;
Yu-long CHEN
5
;
De-xin KONG
5
;
Cheng-zhao WU
1
;
Xiao-fei CHEN
5
;
Zhao-fang BAI
4
;
Ming NIU
4
;
Jia-bo WANG
6
;
Yan-ling ZHAO
4
;
Xiao-he XIAO
4
;
Jin-fa TANG
5
Author Information
1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
3. The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China
4. The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China
5. The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
6. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
- Publication Type:Research Article
- Keywords:
italic>Psoralea corylifolia Linn.;
liver injury;
liver protection;
idney-yin deficiency syndrome;
idney-yang deficiency syndrome;
metabolomics
- From:
Acta Pharmaceutica Sinica
2023;58(4):1014-1023
- CountryChina
- Language:Chinese
-
Abstract:
According to the theory of 'Xingben Dazao' of Psoralea corylifolia Linn. (BL), the susceptible syndromes and biomarkers of liver injury caused by BL were searched. Rat models of kidney-yin deficiency syndrome (M_yin) and kidney-yang deficiency syndrome (M_yang) were established, and all animal experimental operations and welfare following the provisions of the First Affiliated Experimental Animal Ethics and Animal Welfare Committee of Henan University of Traditional Chinese Medicine (No. YFYDW2020017). The results showed that BL significantly decreased the body weight, water intake, and urine weight of M_yin rats and increase the organ indexes of the liver, testis, adrenal gland, and spleen and the expression of alanine aminotransferase (ALT). Meantime, BL significantly increased the urine weight of M_yang rats and decreased the expression of ALT and aspartate aminotransferase (AST). Hematoxylin and eosin (HE) staining showed that BL could aggravate inflammatory infiltration of hepatocytes in rats with M_yin and alleviate liver injury in rats with M_yang. Metabolomics identified 17 BL co-regulated significant differential metabolic markers in M_yin and M_yang rats. Among them, 8 metabolites such as glutamine, quinolinate, biliverdin, and lactosylceramide showed opposite trends, mainly involving cysteine and methionine metabolism, tyrosine metabolism, tryptophan metabolism, purine metabolism, sphingolipid metabolism, glycerol phospholipid metabolism, glutamine metabolism, and other pathways. M_yin/M_yang may be the susceptible constitution of BL for liver damage or protection, which may be related to the regulation of amino acid metabolism and sphingolipid metabolism. The study can provide some experimental data support for the safe and accurate use of BL in the clinical practice of traditional Chinese medicine.
