To explore the quality markers of Huangqin Qingre Chubi capsule in the treatment of rheumatoid arthritis based on network pharmacology combined with pharmacokinetics and target verification
10.16438/j.0513-4870.2022-1447
- VernacularTitle:基于网络药理学结合药动学及靶点验证探究黄芩清热除痹胶囊治疗类风湿关节炎质量标志物
- Author:
Xin-tong DONG
1
;
Jiang-tao KE
1
;
Pei-rong GAN
1
;
Fang-yuan CHEN
1
;
Feng XU
2
;
Jian CHEN
3
;
Jian LIU
4
;
Hong WU
1
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Key Lab of Xin'an Medicine, Ministry of Education, Anhui Province Key Laboratory of Research and Development of Chinese Medicine, Hefei 230012, China
2. The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China
3. School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei 230012, China
4. The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China
- Publication Type:Research Article
- Keywords:
rheumatoid arthritis;
quality marker;
Huangqin Qingre Chubi capsule;
heat arthralgia;
network pharmacology;
pharmacokinetics
- From:
Acta Pharmaceutica Sinica
2023;58(6):1422-1429
- CountryChina
- Language:Chinese
-
Abstract:
As an effective prescription for the treatment of rheumatoid arthritis (RA), Huangqin Qingre Chubi capsule (HQC) is still blank in quality control. This study aims to explore quality markers (Q-markers) for HQC in the treatment of RA by integrating network pharmacology and pharmacokinetics. By constructing the visualization network of "pharmacodynamic ingredient-target-pathway", the potential Q-Marker of HQC treatment for RA was preliminatively predicted. A rat model of rheumatic heat obstruction syndrome collagene-induced arthritis (CIA) was established to elucidate the dynamic quantification law of pharmacodynamic components of HQC in the disease state of rats. To establish the inflammatory model of RA synovial fibroblasts (MH7A) induced by tumor necrosis factor-α (TNF-α) in vitro. The effects of active ingredients on protein expression of sphingosin kinase-1 (Sphk1) and p-SphK1 were detected. The network pharmacological results showed that baicalin, geniposide, luteolin, coixol and amygdalin were the important active components of HQC treatment for RA. Quantitative analysis results further verified the measurability of these five components. The expression of Sphk1 and p-SphK1 was significantly inhibited by geniposide and baicalin by Western blotting. The above studies determined that the above 5 components could be used as Q-markers in the treatment of RA by HQC. This experiment was approved by the Experimental Animal Ethics Committee of Anhui University of Chinese Medicine (approval number: AHUCM-rats-2021049). All procedures were conducted in strict accordance with the principles of animal use and care.
