Quality evaluation of Qilong Zhuang'er oral liquid based on UHPLC-Q-exactive orbitrap MS and chemometrics analysis
10.16438/j.0513-4870.2022-1249
- VernacularTitle:基于UHPLC-Q-exactive orbitrap MS和化学计量学分析的芪龙壮儿口服液质量评价研究
- Author:
Xiao-min CUI
1
;
Ming-zhi DONG
2
;
Hong ZHANG
1
;
Jian-gang LI
3
;
Hui REN
1
;
Jing HU
1
;
Wen-jing LU
1
;
Juan CHEN
1
;
Xue MENG
1
;
Zhi-yong CHEN
1
Author Information
1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710061, China
2. Xi'an C.P. Pharmaceutical Co., Ltd., Xi'an 710043, China
3. Xi'an Peihua University, Xi'an 710199, China
- Publication Type:Research Article
- Keywords:
Qilong Zhuang'er oral liquid;
orbitrap MS;
etermination;
chemometric analysis;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2023;58(6):1655-1662
- CountryChina
- Language:Chinese
-
Abstract:
An UHPLC-Q-exactive orbitrap MS method for the simultaneous determination of 19 chemical components in Qilong Zhuang'er oral liquid was established and the quality differences between different batches of samples was compared by chemometric analysis to provide a basis for the quality evaluation of the preparation. The contents of allantoin, L-proline, pyroglutamic acid, hordenine, adenosine, L-phenylalanine, guanosine, L-tryptophan, caffeic acid, calycosin-7-glucoside, verbascoside, isoacteoside, ononin, calycosin, 3-hydroxy-9,10-dimethoxyptercarpan, formononetin, atractylenolide III, atractylenolide II and astragaloside A were analyzed by cluster heat map, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) using Hiplot platform and MarkerlynxXS software to comprehensively evaluate the quality difference of different batches of Qilong Zhuang'er oral liquid. The 19 chemical compounds showed good linearity in their respective concentration ranges (r ≥ 0.999). The RSD of precision, repeatability and stability (24 h) tests were all less than 1.94%. The average recovery was 97.24%-102.75% (RSD < 2.74%, n = 6). The 10 batches of samples were divided into two categories by cluster heat map and PCA analysis. 3-Hydroxy-9,10-dimethoxyptercarpan, atractylenolide III, calycosin, atractylenolide II, formononetin, allantoin and caffeic acid were identified as differential markers by PLS-DA. The established multi component quantitative method of Qilong Zhuang'er oral liquid combined with chemometric analysis can provide reference for the quality evaluation of the preparation.
