Screening methods of SARS-CoV-2 main protease inhibitors and current applications

VernacularTitle:SARS-CoV-2主蛋白酶抑制剂的筛选方法及应用
Author: Bing YE ; Sheng-hua GAO ; Le-tian SONG ; Yu-sen CHENG ; Mian-ling YANG ; Peng ZHAN ; Xin-yong LIU
Publication Type:Research Article
Keywords: SARS-CoV-2; COVID-19; main protease; rug discovery; high throughput screening
From: Acta Pharmaceutica Sinica 2023;58(6):1528-1539
CountryChina
Language:Chinese
Abstract: COVID-19 epidemic continues to spread around the world till these days, and it is urgent to develop more safe and effective new drugs. Due to the limited P3 biosafety laboratories for directly screening inhibitors of virulent viruses with high infectivity, it is necessary to develop rapid and efficient screening methods for viral proteases and other related targets. The main protease (M^pro), which plays a key role in the replication cycle of SARS-CoV-2, is highly conserved and has no homologous proteases in humans, making it an ideal target for drug development. From two different levels, namely, molecular level and cellular level, this paper summarizes the reported screening methods of SARS-CoV-2 M^pro inhibitors through a variety of representative examples, expecting to provide references for further development of SARS-CoV-2 M^pro inhibitors.