Advances in online two-dimensional liquid chromatography for natural product screening
10.16438/j.0513-4870.2022-1183
- VernacularTitle:在线二维液相色谱技术用于天然产物活性成分筛选中的研究进展
- Author:
Jia-ming YUAN
;
Liang LAI
;
Jin-cai WANG
;
Ting-ting ZHANG
;
Zheng-jin JIANG
- Publication Type:Research Article
- Keywords:
two-dimensional liquid chromatography;
online;
natural product;
active compounds screening
- From:
Acta Pharmaceutica Sinica
2023;58(5):1128-1137
- CountryChina
- Language:Chinese
-
Abstract:
As a treasure resource of novel drug lead compounds, how to rapidly and high-efficiently screen and isolate active components from natural products is critical. Thanks to its high resolution, high automation and flexible integration, online two-dimensional liquid chromatography has great potential for screening active ingredients from complex matrices by integrating a highly specific bio-recognition process into a two-dimensional liquid chromatography system before, on or after the column separation. This review comprehensively summarized recent developments, applications and shortcomings of online two-dimensional liquid chromatography for natural product screening from different integration modes, including pre-column, on-column and post-column screening methods.
