Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS
10.16438/j.0513-4870.2022-1139
- VernacularTitle:基于UPLC-QTOF/MS的茵陈蒿汤体外成分鉴定和急性肝损伤防护作用入血成分研究
- Author:
Yi-qing YAO
;
Qi CAO
;
Xuan WANG
;
Hui-lin MA
;
Yu-miao CHEN
;
Si-yi ZHAO
;
Min-xuan GUO
;
Jia-meng HU
;
Dong-yao WANG
;
Di-ya LÜ
- Publication Type:Research Article
- Keywords:
UPLC-QTOF/MS;
chemical constituent;
blood-absorbed ingredient;
Yin Chen Hao decoction;
acute liver injury;
hepatoprotective effect
- From:
Acta Pharmaceutica Sinica
2023;58(5):1173-1180
- CountryChina
- Language:Chinese
-
Abstract:
To identify the active constituents in vitro and blood-absorbed ingredients in vivo from Yin Chen Hao decoction provides scientific evidence for probing its prevention and treatment mechanism on acute liver injury. An ultrahigh performance liquid chromatography quadrupole-time of flight-mass spectrometry (UPLC-QTOF/MS) method was applied for analysis of Yin Chen Hao decoction and the serum samples of mice with con-A induced acute liver injury after preventive oral administration for 14 days (the use of all laboratory animals in this study was approved by the Ethics Committee of the Naval Medical University, 19YF1459400). A total of 90 chemical constituents were identified from Yin Chen Hao decoction, mainly were flavonoids, terpenoids, tannins, quinones. 5 prototype compounds were identified in the serum, including chrysophanol, deoxyrhapontin-8-O-gallate, mussaenosidic acid, herniarin, emodin. The established UPLC-QTOF/MS method could efficiently and sensitively identify the constituents in vitro and blood-absorbed ingredients of Yin Chen Hao decoction, primarily clarify the material basis of its hepatoprotective effect, and provided a scientific basis for the quality marker selection and the pharmacodynamic material basis research on the decoction.
