Effect of Angelica dahurica coumarins on the transport behavior of puerarin across blood-brain barrier in vitro and in vivo
10.16438/j.0513-4870.2022-1135
- VernacularTitle:白芷香豆素成分对葛根素跨血脑屏障体内外转运的影响
- Author:
Wen-jing TA
;
Ji-hong SONG
;
Cheng-kun HAN
;
Jian-xiang WANG
;
Wen-xue YANG
;
Wen LU
- Publication Type:Research Article
- Keywords:
puerarin;
italic>Angelica dahurica coumarin;
blood-brain barrier;
brain transport;
co-culture cell model
- From:
Acta Pharmaceutica Sinica
2023;57(5):1156-1164
- CountryChina
- Language:Chinese
-
Abstract:
A BBB co-culture cell model consisting of rat brain microvascular endothelial cells (BMEC) and astrocytes (AS) was established to study the effect of Angelica dahurica coumarins on the transport behavior of puerarin across blood-brain barrier (BBB) in vitro and in vivo. The barrier function of this model was evaluated by measuring the transendothelial resistance, phenol red permeability and BBB related protein expression. The permeability assay and western blot methods were performed to study the effects of Angelica dahurica coumarins on the BBB permeability and the expression of BBB related protein. The animal experiment protocols in this study were approved by the Animal Ethics Committee of Xi'an Jiaotong University (Animal Ethics No.: 2021-1329). The results showed that the established BMEC/AS co-culture model could be used to evaluate drug transport across BBB in vitro. After combined with Angelica dahurica coumarins, the transport capacity of puerarin was significantly increased in vitro and in vivo. Additionally, Angelica dahurica coumarins enhanced BBB permeability and inhibited the protein expression of P-glycoprotein (P-gp), zonula occludens-1 (ZO-1) and occludin. Angelica dahurica coumarins might increase BBB permeability by inhibiting the expression of P-gp and tight junction protein, thereby increasing the content of puerarin in brain tissue.
