Identification and analysis of R1-MYB gene family in Rheum palmatum L. based on full-length transcriptome sequencing
10.16438/j.0513-4870.2022-1009
- VernacularTitle:基于全长转录组测序的掌叶大黄R1-MYB基因家族鉴定分析
- Author:
Xia ZHAO
1
,
2
;
Yuan-min LI
1
,
3
;
Yi-min LI
1
,
2
;
Guang-hui XIAO
4
;
Ming-ying ZHANG
1
;
Wen-ping CHENG
1
;
Jing GAO
1
,
2
;
Liang PENG
1
;
Gang ZHANG
1
,
2
Author Information
1. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xi'an 712046, China
2. Key Laboratory for Research and Development of "Qin Medicine" of Shaanxi Administration of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xi'an 712046, China
3. The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xi'an 712046, China
4. College of Life Science, Shaanxi Normal University, Xi'an 710119, China
- Publication Type:Research Article
- Keywords:
italic>Rheum palmatum L.;
R1-MYB;
transcription factor;
gene expression;
stress
- From:
Acta Pharmaceutica Sinica
2023;58(5):1354-1363
- CountryChina
- Language:Chinese
-
Abstract:
As one kind of v-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors, R1-MYB (MYB-related) family plays an important role in plant growth and development, as well as environmental stress and hormone signal transduction. In this study, R1-MYB family genes in Rheum palmatum L. were systematically screened based on full-length transcriptome sequencing analysis. Firstly, the physicochemical, protein domain and molecular evolution characteristics of the coding proteins were analyzed. Furthermore, the tissue expression levels of R1-MYB genes were analyzed by RNA-seq. We also investigated the expression pattern of RpMYB24 in response to various hormones and abiotic stresses. The results showed that a total of 49 R1-MYB genes were identified, which mainly encoded thermally stable hydrophilic proteins. Most of the deduced proteins were predicted to locate in nucleus. Each protein had a large proportion of random curl and α helix, and also had the W-type conserved amino acids which were the signature of MYB. R1-MYB family members were distributed in five subgroups, including circadian clock associated 1 (CCA1)-like, I-box (GATAAG)-like, CAPRICE (CPC)-like, telomere repeat binding factor (TRF)-like and TATA binding protein (TBP)-like, and the number of CCA1-like was the majority. RNA-seq revealed that 49 R1-MYB genes were differentially expressed in roots, rhizomes and leaves of R. palmatum, and the expression levels of 15 and 23 genes in roots and rhizomes were higher than those in leaves, respectively. RpMYB24 transcript was induced by abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA) treatment, and could also significantly respond to injury, low temperature and high temperature stresses except drought stress. This study systematically identified the R1-MYB family genes and their molecular characteristics, better for further gene functional validation, and then provide a scientific basis for the transcriptional regulation mechanism research into rhubarb quality formation.
