Quality evaluation of Bupleuri Radix in Shanxi Province by LC-MS based pseudotargeted metabonomics
10.16438/j.0513-4870.2022-1376
- VernacularTitle:基于LC-MS拟靶向代谢组学的山西北柴胡质量分析
- Author:
Xue BAI
1
;
Ya-xuan GUO
1
;
Ai-ling XU
2
;
Xiao-xia GAO
1
;
Xue-mei QIN
1
;
Xiao-min WANG
2
;
Zhen-yu LI
1
Author Information
1. Modern Research Center of Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. Cotton Research Institute, Shanxi Agricultural University, Yuncheng 044000, China
- Publication Type:Research Article
- Keywords:
Bupleuri Radix;
saikosaponin;
UHPLC QTrap-MS;
pseudotargeted metabonomics;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2023;57(7):1963-1970
- CountryChina
- Language:Chinese
-
Abstract:
Bupleuri Radix is commonly used in the traditional Chinese medicine, and saikosaponins are the important active ingredients. In this study, we first established a relative quantitative method for 25 saikosaponins using ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QTrap-MS) in the scheduled multiple reaction monitoring (sMRM) mode. The established method showed good intra-day and intra-day precision, linearity, repeatability and stability. Then the method was applied to compare 37 batches of Bupleuri Radix from different planting areas. The results showed that there was no significant difference in the saikosaponins composition of Bupleuri Radix from different planting areas in Shanxi Province, which indicating that Bupleuri Radix is well adapted to the environment, so it is suitable for widely planting. However, Bupleuri Radix harvested at spring and autumn were differed from those harvested at summer, which indicated that the traditional harvesting experience was reasonable. Correlation analysis showed that saikosaponins a and d were positively correlated with some saponins, and 4 saponins (such as clinoposaponin XII) showed bigger content variation were identified by coefficient of variation analysis. The LC-MS based pseudotargeted metabonomic method established in this study can be applied to the comprehensive detection of saikosaponins, which providing new method for the quality evaluation of Bupleuri Radix.
