Selection of chemical markers for identification of different species of Juniperri Caulis et Folium based on UPLC-Q-TOF-MS/MS metabonomics technology
10.16438/j.0513-4870.2022-1285
- VernacularTitle:基于UPLC-Q-TOF-MS/MS代谢组学技术筛选不同基原圆柏品种鉴别的化学标志物
- Author:
Zi-wei ZHAO
1
;
Fang PENG
2
;
Kun ZHANG
1
;
Yu-ting LUO
2
;
Yu-jiao ZENG
2
;
Xin-yue WU
2
;
Ce TANG
1
;
Gang FAN
1
Author Information
1. School of Ethnic Medicine of Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. School of Medicine of Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Juniperri Caulis et Folium;
UPLC-Q-TOF-MS/MS;
Metabonomics;
Species identification
- From:
Acta Pharmaceutica Sinica
2023;57(7):1880-1893
- CountryChina
- Language:Chinese
-
Abstract:
In this study, untargeted metabolomics technology based on ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to analyze and identify the overall chemical components of Juniperri Caulis et Folium. Chemical markers for the identification of different Juniperri Caulis et Folium species were screened by integrated principal component analysis and partial least squares discriminant analysis. A total of 58 chemical components were detected and 46 of them were identified, including 26 flavonoids, 8 organic acids and their derivatives, 4 phenylpropanoids, 3 terpenoids, and 5 other components. Among them, methylsyringin and ekersenin were identified for the first time. In the positive ion mode, 12 markers were screened, and in the negative ion mode, 13 markers were screened for species identification. In summary, UPLC-Q-TOF-MS/MS metabonomics technology combined with chemometrics method can effectively reveal the chemical composition differences of different Juniperri Caulis et Folium species, and provide reference for its species identification and quality control.
