The mechanisms of Glycyrrhizae Radix et Rhizoma and its honey-processed products on improving cisplatin-induced acute kidney injury in rats based on metabolomics
10.16438/j.0513-4870.2022-1060
- VernacularTitle:基于代谢组学的甘草及其蜜炙品改善顺铂致大鼠急性肾损伤的作用机制研究
- Author:
Na SUN
1
,
2
;
Min HUA
3
;
Qing YANG
3
,
4
;
Bian-li WANG
3
,
4
;
Rui-xue LIANG
3
,
4
;
Xin-jun ZHANG
3
,
4
;
Xiao-qing YANG
5
;
Jian-yong ZHOU
3
;
Qian ZHOU
3
,
4
,
6
Author Information
1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
2. Shandong Academy of Chinese Medicine, Jinan 250014, China
3. Shandong Academy of Chinese Medicine, Jinan 250014, China
4. Modern Research and Development of Aromatherapy in Traditional Chinese Medicine Shandong Engineering Research Center, Jinan 250014, China
5. Department of Pathology, the First Affiliated Hospital of Shandong First Medical University, Jinan 250014, China
6. The key Unit for Research of technique and principle of Honey-processing and Carbonizing of SATCM, Jinan 250014, China
- Publication Type:Research Article
- Keywords:
raw Glycyrrhizae Radix et Rhizoma;
honey-processed Glycyrrhizae Radix et Rhizoma;
acute kidney injury;
metabonomics;
mechanism
- From:
Acta Pharmaceutica Sinica
2023;58(7):1761-1769
- CountryChina
- Language:Chinese
-
Abstract:
This study used metabolomics to explore the improvement effect of raw and honey-processed Glycyrrhizae Radix et Rhizoma on acute kidney injury (AKI) in rats. All animal experiments were approved by the Animal Ethics Committee of Shandong Academy of Chinese Medicine (approval No.: SDZYY20200101001). SD rats were randomly divided into normal group, model group, raw Glycyrrhizae Radix et Rhizoma group (0.9 g·kg-1) and honey-processed Glycyrrhizae Radix et Rhizoma group (0.9 g·kg-1), 6 rats in each group. The rats model of acute kidney injury was established by single intraperitoneal injection of cisplatin (CP) and treated with raw and honey-processed Glycyrrhizae Radix et Rhizoma. The pathological changes of renal tissue were evaluated by hematoxylin and eosin (HE) and PAS staining, the contents creatinine (Cr), blood urea nitrogen (BUN) and superoxide dismutase (SOD) in serum were detected. UPLC-Q-TOF/MS was used to study tissue metabolomics to screen the biomarkers affected by raw and honey-processed Glycyrrhizae Radix et Rhizoma and analyz the metabolic pathways. The results showed that compared with the model group, raw and honey-processed Glycyrrhizae Radix et Rhizoma can significantly improve the pathological changes of renal tissue and decrease the content of Cr, BUN and increase the activity of SOD. In addition, honey-processed Glycyrrhizae Radix et Rhizoma can also significantly reduce the kidney index. In tissue samples, 45 biomarkers were measured in AKI rats. Raw Glycyrrhizae Radix et Rhizoma and honey-processed Glycyrrhizae Radix et Rhizoma could simultaneously call back 11 differential metabolites, which were involved in the regulation of glycerophospholipid metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and glutathione metabolism. In addition, raw Glycyrrhizae Radix et Rhizoma is also involved in the regulation of glycine, serine and threonine metabolism and pyrimidine metabolism. In summary, raw and honey-processed Glycyrrhizae Radix et Rhizoma can participate in the regulation of different metabolic pathways, and play an improvement role in AKI rats by regulating amino acid, lipid metabolism, energy metabolism and oxidative stress.
