Effect of insulin on the synthesis and secretion of adrenomedullin in rat aortas in vitro
- VernacularTitle:胰岛素对大鼠动脉条分泌肾上腺髓质素的刺激作用
- Author:
Xue-wei XU
;
Chao-shu TANG
;
Ye YANG
;
Xiaoying LI
- Publication Type:Journal Article
- Keywords:
insulin, insulin resistance, glucose, adrenomedullin (ADM)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(6):350-350
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of glucose and insulin on the synthesis and secretion of adrenomedullin (ADM) by rat aortas in vitro.MethodsThe rat aortas were cut into pieces and divided into several equal groups. All the groups were incubated in K-H buffers including different levels of glucose,insulin and insulin+glucose for 3 hours,the group incubated in K-H buffer without glucose was used as control. To determine ADM in K-H buffers and tissues using RIA method.ResultsADM levels in insulin groups (100.0 μIU/ml,200.0 μIU/ml) and insulin+glucose groups were higher than that in control (P<0.05),the ADM levels in glucose groups and low level insulin group (20.0 μIU/ml) were not significantly difference compared with the control. ConclusionHigh levels of insulin can stimulate the synthesis and secretion of ADM.
