Effect of low density lipoprotein and oxidized low density lipoprotein on the secretion of endothelin in endothelial cells
- VernacularTitle:低密度、氧化低密度脂蛋白对内皮细胞分泌内皮素的影响
- Author:
Min ZHANG
;
Xuewei XU
;
Xiaoying LI
- Publication Type:Journal Article
- Keywords:
low density lipoprotein, oxidized low density lipoprotein, endothelial cell, endothelin
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(10):785-786
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of low density lipoprotein(LDL) and oxidized low density lipoprotein(ox-LDL) on the secretion of endothelin(ET) and whether LDL to be oxidized and modified by ox-LDL in vitro.MethodsEndothelial cell strain ECV-304 was incubated with different concentration of LDL(50 and 100 mg/L),ox-LDL(50 and 100 mg/L) or LDL+ox-LDL(50mg/L) for 24 hours,then cells and cultured medium were collected.ET in cells and cultured medium were detected with radioimmunoassay.ResultsBoth of LDL and ox-LDL enhanced the secretion of ET in cultured endothelial cells;the effect of ox-LDL was more markedly than LDL.LDL+ox-LDL had a greater effect than any of the two lipoproteins alone.ConclusionLDL and ox-LDL may have the ability to promote the secretion of ET in cultured endothelial cells and LDL can be oxidized and modified by ox-LDL in vitro.
