Effect of ischemic preconditioning on apoptosis of transplanted pancreas cells in rats
- VernacularTitle:缺血预处理对大鼠移植胰细胞凋亡的影响
- Author:
Xiaonan LIU
;
Tingting HUO
;
Weizhong WANG
;
Wenzian GUAN
;
Dongli CHEN
- Publication Type:Journal Article
- Keywords:
pancreas transplantation, ischemic preconditioning, ischemic reperfusion, apoptosis, rat
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(8):618-620
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of ischemic preconditioning (IPC) on apoptosis of transplanted pancreas cells in rats.Methods6 normal SD rats were assigned as control group. 18 steptozozin-induced diabetic SD rats were randomly divided into 3 groups: the I/R group (n= 6, received pancreas transplantation alone), DIPC group (n=6, received pancreas transplantation exposed IPC with 5 min ischemic and 5 min reperfusion twice) and RIPC group (n=6, received pancreas transplantation exposed IPC with 5 minutes ischemic and 5 minutes reperfusion induced by ligating donors' posterior limbs three times before anastomosing vessel). The blood glucose in serum, superoxide dismutase (SOD), myeloperoxidase (MPO), TUNEL cells in graft were monitored.ResultsAfter reperfusion, compared with the I/R group, the mean blood glucose levels, MPO levels and apoptotice index of graft reduced, the mean SOD levels of graft heightened in DIPC and RIPC groups significantly (all P<0.01).ConclusionIschemic preconditioning induced by graft and ligating donors' posterior limbs can reduce apopotosis of transplanted pancreas cells.
